双酚A及玉米赤霉烯酮对神经母细胞瘤细胞周期蛋白表达的影响

目的 探讨两种环境内分泌干扰物双酚A(bisphenol A,BPA)与玉米赤霉烯酮(zearalenone,ZEA)对人神经母细胞瘤SK-N-SH细胞细胞周期素CyclinD1、CDK4、p21表达的影响.方法 SK-N-SH细胞经培养扩增后分为7组:组1,无干预(对照组);组2,加17β-雌二醇(E2);组3,加...

Effect of bisphenol A and zearalenone on the expression of cell cycle protein in SK-N-SH human neuroblastoma cells

Objective To investigate the effect of environmental endocrine disruptors, bisphenol A (BPA) and zearalenone (ZEA), on the expression of CyclinD1, CDK4 and p21 in SK-N-SH human neuroblastoma (NB) cells in vitro. Methods NB cells were cultured in estrogen free improved minimum essential medium and divided into 7 groups based on different treatments: group 1, no treatment; group 2, treated with 17β-estradiol (E2); group 3, treated with BPA; group 4, treated with zearalenone (ZEA); group 5, treated with both E2 and ICI 182,780; group 6, treated with both BPA and ICI 182,780; group 7, treated with both ZEA and ICI 182,780. Western blot analysis and immunol cytochemistry were employed to monitor the expression of CyclinD1, CDK4 and p21. Results At 72 hours, the expression of CyclinD1 and CDK4 was abundant in 2, 3 and 4 group, but was little in control and 5, 6, 7 group (P<0.05). There was no difference on expression of p21 among the groups (P>0.05). Conclusions Disrupting cell cycle may be involved in the process of growth of SK-N-SH cells, which is directed by BPA and ZEA.