荧光定量分型PCR法与直接测序法检测HBV-DNA水平的应用价值比较

目的比较荧光定量分型PCR法与直接测序法检测HBV-DNA水平的应用价值。方法选取该院2012年1月—2013年12月收治的126例符合入选标准的HBV患者,分别采用荧光定量分型PCR法与直接测序法检测HBV-DNA水平,对HBV-DNA基因分型结果进行比较分析。结果 126份样本只检测出3种基因分型（B型、C型、B/C混合型）,其中B型占明显优势。荧光定量分型PCR法B/C混合型检出率明显高于直接测序法（P〈0.001）;一致性检验表明两种检测方法的一致性较好（Kappa〉0.75）;TA克隆结果与荧光定量分型PCR法一致。结论荧光定量分型PCR法检测HBV-DNA水平应用价值较直接测序法高,值得临床推广应用。

Comparison of the Application Value of Real-time Genotyping and Quanti- tative PCR with That of Direct Sequencing for HBV-DNA Detection

Objective To compare the application value of real-time genotyping and quantitative PCR with that of direct sequenc- ing for HBV-DNA detection. Methods 126 patients with HBV admitted in our hospital and met the inclusion criteria were select- ed. The levels of HBV-DNA of the patients were detected by real-time genotyping and quantitative PCR and direct sequencing, respectively. And the genotyping results of HBV-DNA were compared and analyzed. Results 3 genotypes （B, C, B/C mixed type） were found in 126 samples, the dominant was type B. The detection rate of B/C mixed type by real-time genotyping and quantitative PCR was higher than direct sequencing （P〈0.001）. There was a good consistency of two methods （Kappa〉0.75）. TA cloning results were consistent with real-time genotyping and quantitative PCR. Conclusion The application value of real-time genotyping and quantitative PCR is higher than direct sequencing for HBV-DNA detection, so it is worthy of clinical application and promotion.