华西Ⅲ号液保存鼠肾的实验研究

利用大鼠异体肾移植模型，以ＵＷ液为对照，研究华西Ⅲ号液对鼠肾的保存效果。实验采用封闭群ＳＤ大鼠，体重２００￣２８０ｇ，供受体同性，随机分为对照组（ＵＷ液组）和实验组（ＨＸ－Ⅲ液组）２组。各组又根据保存４８及７２小时分为两个亚组，每个亚组各１０只大鼠。原位灌注切取供体大鼠左肾后低温保存４８、７２小时行异体肾移植。结果：ＵＷ液保存鼠肾４８、７２小时移植后的存活率分别为１００％和９０％；ＨＸ－Ⅲ液保存鼠

A study on the preservation of rat kidney with HX-III solution]

This study was conducted to compare the effects of HX-III solution and UW solution on rat kidney transplantation. Inbred Sprague-Dawley rats weighing 200-280 g, were used in allotransplantation. The donors and recipients were of the same sex. The rats were randomly divided into control (UW solution) and experimental (HX-III solution) groups, and each group included 48- and 72-hour preservation subgroups. There were ten rats in each subgroup. Kidneys were flushed with UW solution or HX-III solution and stored at 4 degrees C for 48 or 72 hours. Then, kidney allotransplantations were performed. The results showed that the rates of survival were 100% and 90% in UW group for 48- and 72-hour cold storage, but those in HX-III were 100% and 60%, respectively. With 48-hour preservation, the maximum serum creatinine levels in UW group were similar to those in HX-III group (P > 0.05). With 72-hour preservation, the maximum serum creatinine levels in UW group were lower than those in HX-III group (P < 0.05). On the 14th day posttransplantation, the histological findings on Kidneys in the two groups for 48-hour cold storage were almost the same, but the grafted kidneys in HX-III group were damaged more seriously than those in UW group for 72 hour cold storage. On the 28th day posttransplantation, the histological findings on the grafted kidneys were basically normal in all groups. The authors concluded that HX-III solution could preserve rat kidney for 48 hours effectively.