乳腺病变的DNA图像定量分析研究

目的：应用自制ＤＮＡ图像分析系统，对５５例乳腺良恶性病变进行测量和研究，评价该软件系统的性能及ＤＮＡ图像分析在乳腺病理诊断中的应用价值。方法：福尔马林固定，石蜡包埋的良、恶性乳腺病变标本经机械及酶消化后，分离成单细胞。涂片行Ｆｅｕｌｇｅｎ染色后进行测量。结果：２９例良性乳腺病变均为二倍体，无１例非整倍体出现（特异性为１００％）；６例非典型增生病变中，有３例为非整倍体；２０例乳腺癌中有１７例非整倍体（敏感性为８５％）。结论：该ＤＮＡ图像分析系统，在乳腺病理诊断中具有辅助诊断意义。

QUANTITATIVE DNA ANALYSIS BY IMAGE CYTOMETRY IN BREAST LESIONS

Objective: 55 cases of breast benign and malignant lesions were studied by DNA image analy-sis system made by ourselves. The function of this analysis system and worth of application of DNA im-age cytometry in pathologic diagnosis of the breast lesions were assessed.Methods:The samples in this experiment were used after fixed in formalin and paraffin embedded.Single cell monolayer smears were made by combined mechanic and enzymatic separation.Feulgen stained smears were measured.Results:29 cases of benign breast lesions were identified as diploidy, no cases of aneuploidy were found speci-ficity 100%).DNA aneuploidy was found in 3 of the 6 cases of atypical hyperplastic lesions and 17 of the 20 cases of breast cancer(sensitivity 85%).Conclusion:This DNA image analysis system has a sig-nificance in aiding pathologic diagnosis in the breast lesions.