不同频率间歇低氧对3T3-L1脂肪细胞炎症因子和脂肪因子的影响

目的:测定不同频率间歇低氧处理的脂肪细胞上清液中肿瘤坏死因子(TNF)-α、白细胞介素(IL)-6和瘦素、脂联素水平的变化.方法:以分化成熟的3T3-L1脂肪细胞为研究对象,随机分为8组,即4个不同频率间歇低氧组(IH1-4,处理方案为分别循环充入1.5％ O245s和21％O22min15s、4min15 s、5min 45 s和8rmin45 s,每组60个循环)和各自的正常氧对照组(SC1～4,分别给予相应时间的21％O2处理).完成暴露后收集培养基,采用酶联免疫吸附(ELISA)法测定脂肪细胞上清液中TNF-α、IL-6、瘦素和脂联素的水平.结果:各间歇低氧组脂肪细胞上清液内TNF-α、IL-6和瘦素的水平均明显高于各自的正常氧对照组(P＜0.05或P＜0.01),且IH3组显著高于其余IH组(P＜0.05或P＜0.01)；各间歇低氧组脂肪细胞上清液内脂联素的水平均明显低于各自的正常氧对照组(P＜0.01),且IH3组显著低于其余IH组(P＜0.05或P＜0.01).结论:间歇低氧可以导致体外培养的脂肪细胞TNF-α、IL-6、瘦素和脂联素的分泌异常,脂肪细胞的内分泌功能紊乱可能参与了阻塞性睡眠呼吸暂停低通气综合征患者的胰岛素抵抗的发生.

Effect of Different Frequency of Intermittent Hypoxia on Proinflammatory Cytokines and Adipokines in 3T3-L1 Adipocytes

Objective: To detect the effect of different frequencies of intermittent hypoxia on levels of tumor necrosis factor (TNF), interleukin (IL) -6, leptin and adiponectin in the supernatant of the adipocytes. Methods: The differentiated 3T3-L1 adipocytes were randomly divided into 8 groups including: four groups treated by different frequencies of intermittent hypoxia (IH1-4, 1.5% O245 s/21%O2 2min15 s, 1.5%O245 s/21%O2 4 min15s, 1.5%O245 s/21%O2 5 min45 s and 1.5%O245 s/21%O2 8 min45 s, respectively, 60 cycles each group), and their sham control groups (SC1-4) exposed to the corresponding period of normal oxygen. The supernatant was collected after finishing exposure and levels of TNF-α, IL-6, leptin and adiponectin were detected by ELISA. Results: Levels of TNF-α, IL-6 and leptin were all significantly higher in intermittent hypoxia group than those of control group (P < 0.05). And levels of TNF-α, IL-6 and leptin were significantly higher in IH3 group than those of other intermittent hypoxia groups (P < 0.05). The adiponectin level was significantly lower in intermittent hypoxia group than that of control group in the supernatant of the adipocytes (P < 0.01), and the adiponectin level was significantly lower in IH3 group than that of other intermittent hypoxia groups (P < 0.05). Conclusion: The intermittent hypoxia was able to induce the abnormal secretion of TNF-α, IL-6, leptin and adiponectin in cultured adipocytes. The endocrine dysfunction of adipocytes was involved in insulin resistance in patients with obstructive sleep apnea-hypopnea syndrome.