PCR-based detection of Bacillus anthracis in formalin-fixed tissue from a patient receiving ciprofloxacin |
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Authors: | Levine Steven M Perez-Perez Guillermo Olivares Asalia Yee Herman Hanna Bruce A Blaser Martin J |
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Affiliation: | Departments of Medicine. Microbiology. Pathology, New York University School of Medicine. Veterans Affairs Medical Center, New York, New York 10016, USA. |
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Abstract: | We demonstrate that Bacillus anthracis may be detected from a formalin-fixed, paraffin-embedded biopsy specimen, even after the patient has received antibiotic treatment. Although traditional PCR methods may not be sufficiently sensitive for anthrax detection in such patients, cycle numbers can be increased or PCR can be repeated by using an aliquot from a previous PCR as the template. |
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