白介素1β和8 mRNA表达等指标监测溃疡性结肠炎活动的应用

目的 研究ＩＬ－１β和ＩＬ－８ｍＲＮＡ的表达,髓化物氧化物酶（ＭＰＯ）和超氧化物歧化酶（ＳＯＤ）活性在监测溃疡性结肠炎（ＵＣ）活动中的作用。方法 分别观察溃疡性结肠炎（ＵＣ）活动期（ＡＵＣ组）患者２０例,ＵＣ非活动期（ＩＵＣ组）患者２３例,非ＵＣ肠道炎症（ＮＵＣ组）患者１４例及对照组患者１２例结肠粘膜活检标本的ＭＰＯ和ＳＯＤ活性及ＩＬ－１β和ＩＬ－８ｍＲＮＡ表达。ＡＵＣ组中的１４例,经治疗２个月病

Application of IL-1 beta and 8 mRNA and some other indices in monitoring the activity of ulcerative colitis

OBJECTIVE: To evaluate the monitoring action of the expression of IL-1 beta, IL-8 mRNA, myeloperoxidase (MPO) and superoxide dismutase (SOD) activity in the active ulcerative celitis (UC). METHODS: Twenty active UC patients, 23 inactive UC patients; and 14 non-UC inflammation patients were selected. Twelve patients who complained of flatulence, abdominal pain and constipation receiving endoscopic examination were used as control. MPO and SOD activity, IL-1 beta and IL-8 mRNA expression (hybridization in situ) were determined on the endoscopic biopsy specimens of all patients. In 20 active UC patients, 14 patients received endoscopic examination and 4 indices detection were done once again after 2 months' treatment with prednisone and SASP. RESULTS: Mucosal MPO activities of active UC patients, inactive UC patients, and non-UC inflammation patients are higher than those of control patients, (19.37 +/- 0.54, 11.59 +/- 1.41, and 12.97 +/- 0.49) U/g tissue vs (9.49 +/- 0.51) U/g tissue (P < 0.01). SOD activities are lower than that of control patients, (5.03 +/- 07,7. 7.66 +/- 0.79, and 6.98 +/- 0.61) U/mg protein vs (8.82 +/- 0.58) U/mg protein (P < 0.05). Mucosal MPO activity of active UC patients is also higher than that of inactive and non-UC inflammation patients (P < 0.01); while SOD activity is lower than them (P < 0.01). After 2 months' medical treatment, MPO activity of 14 active UC patients decreased, (12.61 +/- 0.74) U/g tissue vs (19.31 +/- 0.44) U/g tissue (P < 0.01), while SOD activity elevated (7.44 +/- 0.55) U/mg protein vs (5.10 +/- 1.05) U/mg protein (P < 0.05), compared with that of before treatment. Positive expression of IL-1 beta mRNA appeared in the epithelial and inflammatory cells of all active UC patients, 9 inactive UC patients, and 7 non-UC inflammation patients. While expression of IL-8 mRNA only appeared positively in all active UC patients. In 14 active UC patients, there were no detection of interleukin mRNA expression after 2 months' treatment. CONCLUSIONS: Mucosal MPO, SOD, IL-1 beta, and IL-8 mRNA could be used as 4 indices monitoring the activity of UC. And IL-1 beta mRNA is also helpful to supervise early or late UC activity.