卵巢癌腹水癌细胞端粒酶的检测

目的　探讨卵巢癌患者腹水微量癌细胞中的端粒酶活性检测的临床意义。方法　采用以PCR技术为基础的TRAP方法检测卵巢癌腹水细胞标本中的端粒酶活性,并将检测结果与细胞学诊断结果进行比较。结果　对30例经病理学确诊的卵巢癌腹水细胞标本,采用细胞学检查和端粒酶活性检测发现,20例细胞学阳性腹水标本中,端粒酶阳性19例;7例细胞学阴性腹水标本中,端粒酶阳性3例;3例细胞学可疑腹水标本,其端粒酶活性检测均为阳性。细胞学诊断和端粒酶检测阳性率分别为66.67%(20/30)和83.33%(25/30)。结论　端粒酶活性检测可能在卵巢癌腹腔转移、腹水性质的鉴别诊断和疗后微小残存检测方面有重要辅助诊断价值。

Clinical significance of detecting telomerase activity in ascites cells from patients with ovarian carcinoma

The purpose was to investigate the clinical significance of telomerase activity for tumor diagnosis in ascites cells from patients with ovarian carcinoma. Methods Thirty ascites specimens from patients with ovarian tumor were examined for telomerase activity by PCR based TRAP (telomeric repeat amplification protocol) method, and the result of detection was compared with the cytological diagnosis. Results Telomerase activity was detected in ascites cells from patients with ovarian tumor in 19 of the 20 cytologic possitive specimens and in 3 of the 7 cytologic negative specimens and in all the 3 cytologic suspicious specimens. The positive rate in 30 ascites samples by cytological examination and by the detection of telomerase activity was 66 67% (20/30), and 83 33% (25/30), respectively. Conclusions The result suggested that measuring telomerase activity in ascites cells would be helpful to cytological examination in diagnosis of patients with ovarian carcinoma.