补体活化与中性粒细胞生产活性氧之间的反馈调节作用

目的 在试管实验中证明补体系统的活化与中性粒细胞产生的活性氧间存在相互作用的反馈关系。方法 用菊糖活化的补体作用于中性粒细胞产生活性氧,并进一步通过测定吸光度以观察补体进一步活化情况。用佛波醇肉豆蔻醋酸酯激发中性粒细胞产生的活性氧作用于补体,并进一步采用发光测定仪测定活性氧来观察活经的补体对中性粒细胞释放活性氧的促进作用。结果 试管实验证明活化的补体能刺激中性粒细胞产生活性氧,而活性氧又能进一步活

Relationship between complement activation and reactive oxygen species generation in inflammatory response

OBJECTIVE: To verify the proposed activation feedback mechanism between complement and polymorphonuclear leukocytes(PMN) derived reactive oxygen species(ROS). METHODS: In vitro tests were designed to identify the activation feedback process by alternative activations of complement by inulin or PMNs by 12-O-tetradecanoylphorbol-13-acetate(TPA) in the test medium in consisting serum complement and isolated PMNs, with chemiluminescence technique to determine the production of ROS and complement fixation trial for complement activations. RESULTS: Evidently that activated complement could activate PMNs to release ROS which further activated complement, so the activating feedback cycle was set. CONCLUSIONS: The complement and PMNs-ROS activation feedback cycle mechanism was recognized. It was expected to serve for delineating the regulation of inflammatory response.