| 雷公藤内酯醇对神经胶质瘤侵袭的体外抑制作用 |
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| 引用本文： | 李建斌,翟玉平,祝新根,吕世刚,刘峰,程祖珏. 雷公藤内酯醇对神经胶质瘤侵袭的体外抑制作用[J]. 中国实验方剂学杂志, 2013, 19(9): 260-263 |
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| 作者姓名： | 李建斌 翟玉平 祝新根 吕世刚 刘峰 程祖珏 |
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| 作者单位： | 1. 南昌大学第二附属医院神经外科,南昌,330006
2. 湖口县人民医院神经外科,江西湖口,332500 |
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| 基金项目： | 江西省教育厅项目(EF130) |
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| 摘 要： | 目的:研究雷公藤内酯醇对胶质瘤侵袭性的体外抑制作用,并探讨其作用分子机制.方法:通过MTT法测定雷公藤内酯醇对胶质瘤细胞株U87细胞增殖抑制作用,划痕实验和Transwell实验评价雷公藤内酯醇对细胞侵袭能力的影响,Real-Time PCR和Western印迹法检测雷公藤内酯醇对U87胶质瘤细胞的组织蛋白酶B(cathepsins B,CB)、基质金属蛋白酶9(MMP-9)和核因子NF-κB(p65)表达的影响.结果:雷公藤内酯醇对胶质瘤细胞增殖有显著的抑制作用,同时可降低U87胶质瘤细胞的迁移和侵袭能力,下调其CB,MMP-9和NF-κB表达水平.结论:雷公藤内酯醇降低U87胶质瘤细胞侵袭能力的机制可能与抑制NF-κB通路、下调CB及MMP-9的表达、降低恶性胶质瘤水解细胞外基质的能力有关.
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| 关 键 词： | 雷公藤内酯醇 胶质瘤 U87细胞 侵袭 核因子NF-κB 组织蛋白酶B |
| 收稿时间： | 2012-10-29 |
Anti-invation Effect of Triptolide for U87 Glioma in vitro |
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| LI Jian-bin,ZAI Yu-ping,ZHU Xin-gen,L,#; Shi-gang,LIU Feng and CHENG Zhu-jue. Anti-invation Effect of Triptolide for U87 Glioma in vitro[J]. China Journal of Experimental Traditional Medical Formulae, 2013, 19(9): 260-263 |
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| Authors: | LI Jian-bin,ZAI Yu-ping,ZHU Xin-gen,L&# Shi-gang,LIU Feng CHENG Zhu-jue |
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| Affiliation: | Department of Neurosurgery, the Second Affiliated Hospital of Nanchang University, Nanchang 330006, China;Department of Neurosurgery, Hukou Hospital, Hukou 332500, China;Department of Neurosurgery, the Second Affiliated Hospital of Nanchang University, Nanchang 330006, China;Department of Neurosurgery, the Second Affiliated Hospital of Nanchang University, Nanchang 330006, China;Department of Neurosurgery, the Second Affiliated Hospital of Nanchang University, Nanchang 330006, China;Department of Neurosurgery, the Second Affiliated Hospital of Nanchang University, Nanchang 330006, China |
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| Abstract: | |
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| Keywords: | triptolide glioma U87 cells invasion nuclear factor kappa B callepsins B |
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