他克莫司后处理诱导缺血脊髓对再灌注损伤的耐受*

目的探讨他克莫司后处理能否诱导大鼠缺血脊髓对再灌注损伤的耐受。方法成年雄性SD大鼠30只，随机分为假手术（s0）组、缺血再灌注（IR）组和他克莫司后处理（TP）组，每组10只大鼠，采用经股动脉置管球囊扩张制备脊髓缺血模型，SO组仅行置管，IR组在脊髓缺血20分钟后行再灌注，TP组在脊髓缺血20分钟后再灌注，即刻经左颈总动脉一次性注射他克莫司0．5mg／kg。再灌注后7、14天采用Tarlov评分法检测大鼠后肢运动功能，脊髓组织切片HE染色观察病理学改变。结果SO组大鼠各时间点后肢Tarlov评分均为5分，形态学检测显示脊髓组织结构正常；IR组大鼠Tarlov评分明显降低，脊髓组织呈现出坏死、水肿、空腔形成等缺血再灌注损伤表现；TP组大鼠Tarlov评分结果显著优于IR组，脊髓组织病理变化较IR组为轻。结论建立大鼠脊髓缺血再灌注损伤模型，并初步证实他克莫司后处理能诱导缺血脊髓对再灌注损伤的耐受。

Tolerance of ischemic spinal cord to reperfusion injury induced by tacrolimus postconditioning

Objective To investigate whether tacrolimus postconditioning could induce tolerance of ischemic spinal cord to reperfusion injury in rats. Methods Thirty male SD rats were randomly divided into sham operation （SO） group, ischemia-reperfusion （IR）groupandtacrolimuspostconditioning （TP） group with 10 rats in each group. Themodel of spinal cord ischemia was prepared by means of catheterization through femoral artery and balloon dilatation. SO group received catheterization only. IR group underwent reperfusion 20 minutes after spinal cord ischemia. TP group experi- encedasingleinjectionoftacrolimus （0.5mg/kg） throughtheleftcommoncarotidarteryattheonsetofreperfusion. At 7 and 14 days after reperfusion, hindlimb motor function was detected by using Tarlov scale and pathological changes in spinal cord tissue sections were observed through HE staining. Results In SO group, Tarlov scores were 5 and the spinal cord tissues remained intact at each time point. Tarlov scores of IR group dropped remarkably and the spinal cord tissues demonstrated lesions such as necrosis, edema and cavity formation. Tarlov scores were significantly higher in TP group than in IR group, and pathological changes in TP group were not as serious as those in IR group. Conclusion We suc- cessfully establish the model of spinal cord ischemia-reperfusion injury in rats and initially confirm that tacrolimus post- conditioning can induce tolerance of ischemic spinal cord to reperfusion injury.