白术对小鼠小肠推进功能的影响研究

目的建立评价小鼠小肠推进功能的实验方法并考察不同白术的药效作用。方法以小鼠小肠碳末推进率为指标,对给药浓度、给药体积、禁食条件进行考察。结果以2%碳墨汁为内标物,禁食不禁水12 h,白术生药浓度为0.5g/ml,按0.15 ml/g体重灌胃给药,给药后30 min脱颈处死小鼠条件下,小鼠小肠推进效果最佳。结论建立的实验方法能够客观评价小鼠小肠推进功能,为白术的药效评价提供实验依据。

STUDY THE EFFECT OF ATRACTYLODES MACROCEPHALA ON PROMOTING SMALL IN- TESTINE FUNCTION IN MICE

Objective To establish the experimental method to evaluate the effect of Artactylodes macro- cephala on promoting small intestine propelling function in mice. Methods Take carbon propelling in small intestine as the mark, the conditions include the volume, the concentration and the condition of administration were observed. Results The optimal method was using 2% ink with carbon powder as an internal standard, the mice were fasted 12 hours, but water was given, the best concentration of Artactylodes macrocephala was 0.5g/ml, the volume was 0. 15 ml/g（ by body weight）. 30 minutes after administration mice were put to death, and then the effect on intestine pro- pelling will be the best. Conclusion The optimal method established can objectively evaluate the effect of Artac- tylodes macrocephala on promoting small intestine propelling function in mice.