| 藏药材猪殃殃质量标准研究 |
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| 引用本文： | 陈燕,刘倩伶,德吉,刘云华,易进海.藏药材猪殃殃质量标准研究[J].中国民族医药杂志,2009,15(5):40-42. |
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| 作者姓名： | 陈燕 刘倩伶 德吉 刘云华 易进海 |
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| 作者单位： | 1. 四川省中医药科学院,成都,610041
2. 西藏自治区藏医医院,拉萨,850000 |
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| 摘 要： | 目的：建立猪殃殃药材中绿原酸的薄层色谱鉴别与含量测定方法。方法：采用TIE法对猪殃殃进行鉴别；采用HPLC法测定绿原酸含量，色谱条件为：色谱柱：Phenomenex Luna C18（4．6mm×150mm，5μm）；流动相：乙腈-0．4％磷酸溶液（10：90）；检测波长：327nm；流速：1．0ml/min；柱温：35℃。结果：各批猪殃殃药材TLC色谱中均能检出绿原酸；绿原酸眦色谱峰与其它色谱峰分离良好，进样量在0．0892-0．4460μg范围内，呈良好线性关系（r=0．9997），平均回收率为96．99％，RSD=1．17％（n=6）。结论：本法简便快捷，结果可靠．为控制猪殃殃药材的质量提供参考。
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| 关 键 词： | 猪殃殃 薄层色谱鉴别 绿原酸 高效液相色谱法 |
Study on Quality Control of Galium aparine |
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| Chen Yan,Liu Qian-ling,Deji,Liu Yu-hua,Yi Jin-hai.Study on Quality Control of Galium aparine[J].Journal of Medicine & Pharmacy of Chinese Minorities,2009,15(5):40-42. |
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| Authors: | Chen Yan Liu Qian-ling Deji Liu Yu-hua Yi Jin-hai |
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| Institution: | Chen Yan, Liu Qian - ling, Deji, Liu Yu - hua, Yi Jin - hai( Sichuan Academy of Chinese Medicine Sciences(Chengdu610041 )) |
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| Abstract: | Objective： To establish methods for determination and the TLC identification of chlorogenic acid in Galium aparine. Methods：TLC and HPLC were adopted to identify and determine Galium aparine. The chromatographic conditions were： Phenomenex lama C18 （2）, （4.6mm × 150mm,5μm）column as analytic column; Acetonitrile- 0.4%H3PO4 water solution （10：90） as mobile phase; detection wavelength at 327nm; column temperature at 35℃ ;flow rate at 1. 0ml· min^- 1. Results： chlorogenic acid was identified by TLC and determined by HPLC. The liner range of chlorogenic acid was 0. 0892 - 0. 4460μg, r = 0. 9997. The average, recovery was 96.99%, RSD = 1.17%. Conclusion： These methods are simple, accurate, reproducible and can be used to enhance the quality control of this drug. |
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| Keywords: | Galium apafine TLC identification Chlorogenic acid HPLC determination |
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