| 细胞培养酶免疫法在检测和分析大肠癌细胞McAb中的应用 |
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| 引用本文： | 汪军,林彩霞,俞慧,李洸. 细胞培养酶免疫法在检测和分析大肠癌细胞McAb中的应用[J]. 中国免疫学杂志, 1988, 0(2) |
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| 作者姓名： | 汪军 林彩霞 俞慧 李洸 |
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| 作者单位： | 浙江省医学科学院 杭州(汪军，林彩霞，俞慧)，浙江省医学科学院 杭州(李洸) |
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| 摘 要： | 由于细胞表面抗原的提纯较难,检测抗细胞类抗原McAb常采用全细胞作抗原。一些学者多采用细胞悬液离心干燥法制备全细胞固相抗原,用免疫酶法进行McAb检测。由于细胞悬液包底,不均匀,以致影响结果的观察。本文报道在制备抗大肠癌HR_(8348)细胞McAb中,将HR_(8348)细胞直接培养于微板孔中,利用其贴壁单层生长的特性,制成包被均
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APPLICATION OF CELL ENZYME IMMUNOASSAY FOR DETECTION AND ANALYSIS OF MONOCLONAL ANTIBODIES TO HUMAN COLORECTAL ADENOCARCINOMA |
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| Wangjun,Yuhui,Lin Caixia,Liguang. APPLICATION OF CELL ENZYME IMMUNOASSAY FOR DETECTION AND ANALYSIS OF MONOCLONAL ANTIBODIES TO HUMAN COLORECTAL ADENOCARCINOMA[J]. Chinese Journal of Immunology, 1988, 0(2) |
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| Authors: | Wangjun Yuhui Lin Caixia Liguang |
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| Abstract: | A solid-phase cell enzyme-linked immunoassay is described for screeningand analyzing monoclonal antibodies against the cell surface antigensof human colorectal adenocarcinoma.Horse-radish peroxidase conjugatedProtein A was used to detect the binding of mouse monoclonal antibodiesto human colorectal adenocarcinoma.HR_(8348) cells which had been cultured inand then fixed to the wells of microtiter plates by using glutaraldehyde.Thismethod was found to be specific,reproducible and practical,and especiallyto be advantageous for the large scale screening and analyzing of monoclo-nal antibodies with a panel of cell types. |
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| Keywords: | Cell Enzyme Assay Human Colorectal Adenocarcinoma. |
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