恶性肿瘤DNA倍体异质性与细胞生物学特性的关系

目的 探讨恶性肿瘤组织DNA倍体异质性与癌细胞生物学特性的关系.方法 采用流式细胞术(FCM)检测163例恶性肿瘤组织DNA倍体异质性,并分析DNA倍体异质性与肿瘤细胞DNA指数(DI)、细胞凋亡(Apo)百分比、S期细胞百分比(SPF)以及DNA倍体类型等细胞生物学特性参数之间的相关性.结果 各种恶性肿瘤的DNA倍体异质性明显不同,在6种肿瘤中,DNA倍体异质体检出率大小分布顺序与DNA异倍体的分布十分相似.恶性肿瘤组织的DI值与DNA异质体检出率呈显著正相关(r=0.872 4,P＜0.05).异质体的DNA异倍体检出率(100.0%)和SPF(1.51±0.67)%1均显著高于同质体22.0%,(0.34±0.51)%](P均＜0.01),而Apo百分比(2.08±1.25)%]显著低于同质体组织(11.99±10.25)%](P＜0.01).结论 DNA倍体异质性是恶性肿瘤的重要生物学特性之一,且与癌细胞其他生物学特性关系十分密切,可作为肿瘤的恶性程度和预后判断的重要指标.

Relationship between DNA ploidy heterogeneity and biological characteristics of cancer cells on patients with malignant tumors

Objective　To explore the relationship between DNA ploidy heterogeneity and biological characteristics of cancer cells on patients with malignant tumor. Methods　The DNA ploidy heterogeneity of tumor tissue was measured on 163 patients with malignant tumors by flow cytometry. The relations were analyzed between DNA ploidy heterogeneity and DNA index(DI), percentage of heteroploid, parameters of cellular kinetics from patients with malignant tumors. Results　The percentage of DNA ploidy heterogeneity of 6 tumors differed obviously, the order of which similar with that of DNA heteroploid. There was a significant positive correlation between DI value and the percentage of DNA ploidy heterogeneity(r = 0.872 4, P < 0.05). Both the percentage of DNA heteroploid(100.0 % vs 22.0 %) and SPF(1.51±0.67)% vs (0.34±0.51)%] of heteroplasmon were higher than those of homoplasmon significantly (P <0.01), but the reverse the percentage of cell apoptosis(2.08±1.25)% vs (11.99±10.25)%]（P <0.01）. Conclusion　As one of the important biological characteristics of malignant tumors, DNA ploidy heterogeneity is closely related to other biological characteristics and may be regarded as a judgment marker of malignant degree and prognosis of tumors.