乙型肝炎分子流行病学分析

目的探讨乙型肝炎在患者及其子女间的遗传传递。方法对乙型肝炎患者３５个家系７４个个体，进行了血清游离型和外周血单核细胞内整合型ＨＢＶＤＮＡＵ５样序列片段和单链ＤＮＡ构象多态性等指标检测。结果男性患者及其病后出生的子女Ｕ５样序列检出率均显著高于其病前出生子女，差异有显著性；Ｕ５样序列检出率在乙型肝炎患者和该患者病后出生的子女呈一致性增高，可具有完全一致的单链泳动变位。男性患者病后出生子女的ＨＢｓＡｇ、ＨＢｅＡｇ、抗－ＨＢｃ阳性率和感染率均分别显著高于其病前出生子女，差异有显著性；男性患者的ＨＢｓＡｇ、ＨＢｅＡｇ、抗－ＨＢｃ阳性率与其病后出生子女呈一致性增高。结论从分子遗传学水平为乙型肝炎的遗传传递学说提供了证据。

Molecular Epidemiologic Analysis of Hepatitis B

Objective To study genetic transmission of hepatitis B (HB) between the patients and their children. Methods Serum free HBV DNA U5 sequence fragment, integrated type of it in peripheral mononuclear cell and configuration polymorphism of single strand DNA were detected in 74 individuals from 35 families. Results It showed that frequency of U5 sequence fragment was significantly higher in male patients and their children born after the occurrence of hepatitis B than in those born before it. They had the completely same electrophoresis sites of single strand DNA. Positive rate and infection rate of hepatitis B surface antigen (HBsAg), hepatitis Be antigen (HBeAg) and core antibody against hepatitis B were all significantly higher in the children born after the occurrence of the disease of male patients than those born before it. Conclusion It provides evidence for the theory of genetic transmission of hepatitis B on the basis of molecular genetics.