铁补充对大鼠肝脏铁与叶酸代谢的影响

目的研究缺铁性贫血时机体内叶酸含量的变化及其与铁补充的关系。方法ＳＤ纯品系雄性断乳大鼠３２只，随机分为铁正常、铁缺乏两组。喂纯合成饲料，铁含量分别为８００μｇ与小于２０μｇ，每日两次定时喂饲。两组模型建立后再各取一半大鼠每日补充铁剂８０００μｇ，同前方式每日两次喂饲；另一半大鼠继续原方案不变，共建立铁正常补铁组、铁缺乏补铁组与铁正常、铁缺乏对照４个组；４个实验组每日饲料叶酸含量均小于１０μｇ。结果铁缺乏影响大鼠的一般生长，体重与血红蛋白含量；肝脏重量与肝内铁含量明显低于正常水平，而叶酸含量明显高于铁正常大鼠。补铁后，铁缺乏大鼠的生长、体重、血红蛋白以及肝脏重量明显改善，肝脏的铁含量迅速增加并超出正常水平。在肝脏铁增加的同时，肝脏组织的叶酸含量明显下降，直至显著低于正常。结论提示铁与叶酸同时缺乏的大鼠在补充大剂量铁剂后导致机体内叶酸含量进一步减少，而铁正常大鼠上述变化不明显。

Effects of Iron Supplement on Metabolism of Iron and Folic Acid in Rat Liver

OBJECTIVE: To study the effects of iron supplement on metabolism of iron and folic acid. METHODS: Thirty-two weanling Sprague-Dawley rats were divided into two groups, iron-normal and iron-deficiency, feeding twice a day with synthetic fodder containing 800 micrograms and less than 20 micrograms iron per day, respectively. Half of the rats in each group were supplemented with 8,000 micrograms of iron daily, and the other half of the rats were fed unchanged. Finally, four groups of rats, i.e., iron-normal with iron supplement, iron-deficiency with iron supplement, iron-normal and iron-deficiency, were set up. The fodder for all the four groups contained less than 10 micrograms of folic acid per day. RESULTS: Iron deficiency affected the growth, body weight and hemoglobin level of the rats, and their liver weight and iron content in the liver were significantly lower than the normal level, and their folic acid level was significantly higher than that in iron-normal rats. The growth, weight and hemoglobin level improved significantly, and iron content in the liver increased rapidly and exceeded the normal level after supplement with iron, while folic acid level in the liver declined until that was significantly lower than the normal. CONCLUSION: Folic acid content in the rats with deficiency both in iron and folic acid declined further after iron supplement, and no significant change was found in iron-normal rats.