人造矿物纤维对肺巨噬细胞的毒作用研究

比较１０种人造矿物纤维对肺泡巨噬细胞毒作用的差异。方法选用日本纤维状物质研究匠１０种标准人造造矿物纤维，在体外用豚鼠肺泡巨噬细胞作用靶细胞，分别对ＪＦＭ纤维作用于ＡＭ后超氧阴离子自由基、过氧化氢的产生，谷胱甘肽及细胞内游离Ｃａ＾２＋浓度的变化进行了测定。

Studies on cytotoxicity of man-made mineral fiber to alveolar macrophage]

OBJECTIVE: To compare the difference in cytotoxicity to human alveolar macrophage caused by ten kinds of man-made mineral fiber. METHODS: Ten kinds of man-made mineral fiber prepared by Japan Fibrous Materials Research Association (JFM) were used with alveolar macrophage extracted from guinea pigs in vitro as the target cells. Changes in the production of O2- and H2O2, levels of glutathione and cellular free calcium ion (Ca2+) in alveolar macrophage were determined. RESULTS: Most of the 10 kinds of JFM fiber could cause increased production of O2- and H2O2, reduction of levels of glutathione and increase of cellular free calcium ion (Ca2+) in alveolar macrophage, to a extent less than that caused by chrysotile. CONCLUSION: In general, the cytotoxicity of JFM fiber was less than that of chrysotile. Although these mineral fiber could be used as substitutes for asbestos, they still could cause certain harm to human bodies.