非甾体类抗炎药对荷瘤小鼠肝癌的促凋亡作用（英文）

目的:探讨非甾体类抗炎药(NSAIDs)中选择性及非选择性的环氧化酶-2抑制剂对小鼠移植的肝肿瘤的凋亡诱导作用和其分子机理。方法:昆明种小鼠腋下无菌接种腹水保种的鼠肝癌H22细胞, 给予布洛芬, 吲哚美辛和尼美舒利灌胃每天1次, 21 d后断颈法处死小鼠, 应用电镜, DNA ladder, 流式细胞术及Western blot等方法研究NSAIDs对小鼠荷瘤的作用及分子机理。结果:3种药均能诱导Hep22肿瘤细胞的凋亡, 与对照组(3.3%±0.6%)相比, 布洛芬, 吲哚美辛和尼美舒利的细胞凋亡率分别上升为15%±1.0%, 29.7%±1.5%和46.3%±3.5%。尼美舒利治疗组呈现更为明显的凋亡形态和DNA梯度, 也明显下调bcl-x_L 和bcl-2的表达, 而吲哚美辛和布洛芬下调bcl-2表达的同时上调bcl-x_L的表达。3种药物都不改变c-myc的表达。结论:NSAIDs可抑制小鼠肝肿瘤的生长, 诱导其肿瘤细胞调亡, 其中选择性COX-2抑制剂尼美舒利的作用最为明显, 并与调节bcl-x_L 和bcl-2的表达有关。

Non-steroidal anti-inflammatory drugs induce apoptosis in implanted hepatoma in mice

AIM: To study the mechanism of the effect of NSAIDs on apoptosis in mice hepatomaat anti - inflammatory doses. METHODS: Kunming breed mice were inoculated subcutaneously in the flankwith mice hepatoma H22 cell line. The effects of ibuprofen, indomethacin, and nimesulide on apoptosis weredetermined by using electron microscopy, agarose gel electrophoresis, flow cytometry, and Western blot analy-sis of the expression of c - myc, bcl - xL and bcl - 2 proteins. RESULTS: NSAIDs induced apoptosis of miceimplanted hepatoma, which includes the morphological changes such as reduction in the volume, and the nu-clear chromatin condensation, as well as the "ladder pattern" revealed by agarose gel electrophoresis of DNA.The apoptotic index was increased to 15.0% + 1.0%, 29.7% + 1.5%, 46.3% + 3.5% from 3.3% +0.6 % by detecting Sub - G1 peaks on flow cytometry. Western blot analysis showed that levels of bcl - 2 andbcl - xL were significantly reduced by treatment with nimesulide. Ibuprofen and indomethacin decreased bcl -2 expression but increased bcl - xL expression. C - myc wasn' t changed in these groups. CONCLUSION:These results suggest that NSAIDs induce apoptosis of mice hepatoma, which may be due to their regulation onthe expressions of bcl - 2 family genes.