过氧亚硝基阴离子在肝纤维化形成中的作用

目的：探讨过氧亚硝基阴离子（ONOO-）在肝纤维化形成过程中所起的作用。 方法： 采用复合因素建立肝纤维化（HF）动物模型的同时，用一氧化氮合酶抑制剂L-NNA（20 mg·kg－1·d－1）给大鼠灌胃（HF+NNA），于实验4周末测定血浆脂多糖（LPS）、NO2-/NO3-含量；采用免疫组化的方法检测肝组织硝基酪氨酸（NT）的表达；用VG染色观察肝组织纤维化程度。 结果： 在HF＋NNA组血浆NO水平明显低于HF组（P＜0.01），HF组NT表达最强，纤维化程度最严重。 结论： ONOO-能促进肝纤维化的发展。

Effect of peroxynitrite anion (ONOO-) on the development of hepatic fibrosis

AIM: To explore the effect of peroxynitrite anion (ONOO-) on development of hepatic fibrosis. METHODS: The hepatic fibrosis (HF) of Wistar rats was established by giving complex pathogens. The rats were intra-gastrically infused with 0.9% salt (1.5 mL/d) and inhibitor of nitric oxide synthase (NOS), L-NNA (20 mg·kg－1·d－1), respectively. At the end of the 4th week, the levels of plasma LPS and NO2-/NO3- were detected. The expression of nitrotyrosine (NT) in hepatic tissue was examined by immunohistochemical techniques. The degree of hepatic fibrosis was observed by VG staining. RESULTS: The level of plasma NO in HF+NNA group was remarkably lower than that in HF group (P<0.01). The expression of NT in hepatic tissue was the strongest and the degree of hepatic fibrosis was the most serious in HF group. CONCLUSION: ONOO- promotes the development of hepatic fibrosis.