400例健康体检妇女宫颈感染人乳头瘤状病毒基因亚型的检测及分析

目的了解健康体检妇女宫颈人乳头瘤状病毒(human papillomavirus,HPV)感染状况和亚型分布,为宫颈癌的防治提供理论依据。方法对体检女性宫颈脱落细胞应用基因芯片技术进行HPV亚型检测并分析其感染率及年龄分布。结果400例女性中共检出HPV阳性68例,占总检测人数17.0%。68例中高危型HPV感染者42例,占61.8%;低危型HPV感染者15例,占22.1%;高低危混合HPV感染者11例,占16.1%。阳性患者中高危型以16型为主,占23.5%;其次为66型,占8.8%;低危型以43和11型为主,分别占13.2%和8.8%。在各年龄组中,20～34岁妇女HPV感染率最高,为18.2%,≤19岁、≥35岁患者的HPV感染率依次为15.4%和12.7%,受检者各年龄组之间HPV感染率差异无统计学意义。结论健康体检女性中有相当的比例感染HPV,尤其是高危型。对体检女性进行HPVDNA检测及分型有重要意义。

Prevalence and Genotypes of Human Papillomavirus Cervical Infection in 400 Women Who Underwent Health Checkup

Objective To evaluate prevalence of human papillomavirus(HPV) infection and genotypes of the virus in 400 women who underwent health checkup. Methods HPV DNA was determined from the cervical epithelium of 400 women by means of gene chip technique. The overall HPV prevalence and age distribution in the 400 women were analyzed. Results HPV DNA was detected in 17.0% of all the women(68 of 400 cases), The high risk HPV was detected in 61.8% of the HPV-positive women(42 of 68 cases) and HPV 16 was the most common type. The low risk HPV was detected in 22.1% of the HPV-positive women(15 of 68 cases), HPV43 and HPV 11 were the most common types. The combined high and low risk HPV was detected in 16.1% of the HPV-positive women(11 of 68 cases). The highest HPV prevalence was found in women at the ages of 20~34 years(18.2%) compared with the women aged ≤19 years(15.4%) and aged ≥ 35 years(12.7%) , but there were no significant differences among the age groups. Conclusion Gene chip technique detects a high prevalence of HPV in the women who underwent health checkup.