Determination of olprinone in human plasma utilizing liquid chromatography tandem mass spectrometry |
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Authors: | Ping Zhu Yu-Guan Wen Ji-Mei Chen Jian Zhuang Zhi-Ling Zhou Shao-Yi Zheng Ruo-Bin Wu Xue-Jun Xiao Cong Lu Rui-Xin Fan Hui-Ming Guo Xiao-Ping Fan |
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Affiliation: | 1. Department of Cardiovascular Surgery, Guangdong Cardiovascular Institute, Guangdong General Hospital, Guangdong Academy of Medical Sciences, 96 Dongchuan Road, Guangzhou 510080, People''s Republic of China;2. Department of Clinical Pharmacology, Guangzhou Brain Hospital, Guangzhou 510370, People''s Republic of China |
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Abstract: | A sensitive and rapid method was developed for quantification of olprinone in human plasma utilizing liquid chromatography tandem mass spectrometry (LC–MS/MS). An aliquot of 1 mL plasma sample was extracted with ethyl acetate–dichloromethane. Separation of olprinone and the milrinone (internal standard, IS) from the interferences was achieved on a C18 column followed by MS/MS detection. The analytes were monitored in the positive ionization mode. Multiple reaction monitoring using the transition of m/z 251 → m/z 155 and m/z 212 → m/z 140 was performed to quantify olprinone and IS, respectively. The method had a total chromatographic run time of 3 min and linear calibration curves over the concentration range of 0.5–60 ng/mL. The lower limit of quantification (LLOQ) was 0.5 ng/mL. The intra- and inter-day precisions were less than 16.3% for low QC level, and 7.1% for other QC levels, respectively. The intra- and inter-day relative errors were ranged between −12.2% and 3.7% for three QC concentration levels. The validated method was successfully applied to the quantification of olprinone concentration in human plasma after intravenous (i.v.) administration of olprinone at a constant rate of infusion of 2 μg/(kg min) for 5 min in order to evaluate the pharmacokinetics. |
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Keywords: | Olprinone LC&ndash MS/MS Pharmacokinetics Plasma concentration Quantitative assay |
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