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| 过表达p27基因抑制血管损伤后新生内膜的形成 | |
| 引用本文： | 田清平,霍勇,郭艳红,孟磊,王贵松,喻卓,吴小兵,高炜.过表达p27基因抑制血管损伤后新生内膜的形成[J].中国介入心脏病学杂志,2004,12(5):305-308. |
| 作者姓名： | 田清平 霍勇 郭艳红 孟磊 王贵松 喻卓 吴小兵 高炜 |
| 作者单位： | 1. 100034,北京大学第一医院心内科 2. 北京大学第三医院心内科 3. 本元正阳公司基因技术股份有限公司 |
| 基金项目： | 北京市自然科学基金资助(7032027)，教育部教育振兴行动计划特殊专项(九八五工程)资助 |
| 摘 要： | 目的 探讨p2 7基因转染对血管损伤后新生内膜形成的影响。方法 以腺病毒为载体转染p2 7基因 ,用MTT法观察其对血管平滑肌细胞增殖的影响 ,用流式细胞仪检测细胞周期 ;在兔颈总动脉球囊损伤模型上转染p2 7基因 ,采用免疫组织化学法检测p2 7基因的表达 ,检测其对新生内膜形成的影响。结果 p2 7可明显抑制培养的血管平滑肌细胞增殖 ,细胞停滞于G1期。兔颈总动脉球囊损伤后转染p2 7基因可以减少新生内膜的形成达 2 7 4 3%。结论 以腺病毒为载体转染p2 7基因可以有效地抑制血管平滑肌细胞增殖及血管损伤后新生内膜的形成。 |
| 关 键 词： | 基因 p27 血管内膜 |
| 修稿时间： | 2004年3月24日 |
Overexpression of p27 gene inhibits the neointimal formation after balloon injury in rabbit |
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| TIAN Qingping,HUO Yong,GUO Yanhong,et al..Overexpression of p27 gene inhibits the neointimal formation after balloon injury in rabbit[J].Chinese Journal of Interventional Cardiology,2004,12(5):305-308. | |
| Authors: | TIAN Qingping HUO Yong GUO Yanhong |
| Institution: | TIAN Qingping,HUO Yong,GUO Yanhong,et al. Department of Cardiology,Peking University First Hospital,Beijing 100034,China |
| Abstract: | Objective To evaluate the function of p27 gene on the neointimal formation after balloon injury in rabbit carotid artery. Methods The cultured vascular smooth muscle cells (VSMCs) were infected with Adp27. Using MTT assay we measured the proliferation of VSMCs. Balloon injured rabbit carotid arteries were also infected with Adp27, and then we evaluated the ratio of intima to media. Results MTT assay of rabbit VSMCs proliferation demonstrated an obvious inhibition in Adp27 treated group. And p27 overexpression had an effect on the cell cycle. It could induce cell cycle arrest in G1 phase and reduce the cells in S phase. Overexpression of p27 could also reduce the neointimal hyperplasia by 27.43%. Conclusion The p27 gene can reduce the neointimal formation after balloon injury in rabbit carotid artery effectively. |
| Keywords: | Genes p27 Tunica intima |
| 本文献已被 CNKI 万方数据 等数据库收录！ | |