| 人促甲状腺激素受体的表达及其抗体的检测 |
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| 引用本文： | 王少艳,冯凭,方佩华.人促甲状腺激素受体的表达及其抗体的检测[J].国际内分泌代谢杂志,2006,26(3):178-180. |
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| 作者姓名： | 王少艳 冯凭 方佩华 |
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| 作者单位： | 1. 300052,天津医科大学总医院代谢病科
2. 300052,天津医科大学总医院核医学科 |
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| 摘 要： | 人促甲状腺激素受体(TSHR)是自身免疫性甲状腺疾病(AITD)的一个重要抗原,TSHR抗体(TRAb)的检测对AITD的鉴别诊断及评价预后非常重要。目前TRAb的检测方法主要有两种,即放射受体分析法和生物分析法。两种方法均需要足够量的高纯度、高活性的TSHR蛋白,分子生物学的发展使重组TSHR的产量和活性大为提高,报告基因的引入可以区别甲状腺刺激性抗体和甲状腺阻滞性抗体。
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| 关 键 词： | TRAb TSHR 自身免疫性甲状腺疾病 |
| 收稿时间： | 2005-10-31 |
| 修稿时间： | 2005年10月31 |
The expression of human thyrotropin receptor and the detection of thyrotropin receptor antibody |
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| WANG Shao-yan,FENG Ping,FANG Pei-hua.The expression of human thyrotropin receptor and the detection of thyrotropin receptor antibody[J].International JOurnal of Endocrinology and Metabolism,2006,26(3):178-180. |
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| Authors: | WANG Shao-yan FENG Ping FANG Pei-hua |
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| Institution: | Department of Metabolism, The General Hospital of Tianjin Medical University, Tianjin 300052, China |
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| Abstract: | Human thyrotropin receptor(TSHR) is an important antigen of autoimmune thyroid disease(AITD). The detection of TSHR antibody(TRAb)play an important role in the diagnosis and prognosis evalutaion of AITD. At present, two detection techniques, radio-receptor assay and bioassay, are usually used to detect TRAb. Both assays need plenty of high-purified and high-active protein. With the development of molecular biology, the yield and activity of recombinant TSHR are improved greatly. The use of report gene make it possible to distinguish thyroid stimulating antibody and TSH-binding antibody. |
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| Keywords: | TRAb TSHR Autoimmune thyroid disease |
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