瘦素基因启动子区-2548G/A多态性与非酒精性脂肪肝的相关分析

目的观察瘦素基因变异(启动子区-2548G/A)与非酒精性脂肪肝病易感性的关系。方法用PCR-RFLP方法,对223例广州市城区居民,其中非酒精性脂肪肝病组108例,对照组115例,进行基因多态性分析。结果①在女性非酒精性脂肪肝组的GA、GG基因型频率及等位基因频率明显高于对照组(2=8·254,p=0·011),而男性各组的基因分布频率改变不明显(2=3·810,p=0·316)。②血清瘦素水平受性别及等位基因变异的影响。结论①瘦素基因变异对广东汉族人群非酒精性脂肪肝易感性的影响可能存在着性别的差异。②含瘦素基因-2548G/A等位基因G的血清瘦素水平比含等位基因A的明显增高。

ASSOCIATION OF -2548G/A POLYMORPHISM IN THE PROMOTER REGION OF LEPTIN GENE WITH NONALCOHOLIC FATTY LIVER DISEASE

Objective To observe association of -2548G/A polymorphism in the promoter region of leptin gene in patients with the susceptibility of nonalcoholic fatty liver disease. Methods A total of 223 city dwellers in Guangzhou, including 108 NAFLD, and 115 health controls were recruited. The polymorphism of leptin gene was determined with PCR-RFLP technique. Results ①Both genotype and allele frequencies of leptin gene 2548 G/A(GA,GG) in women with NAFLD were significantly higher than those in normal group(~ 2 =8.254,p=0.011). But there is not significant difference between two groups in male subjects(~ 2 =3.810,p=0.316). ②There was a significant interaction between sex and genotype on letin levels. Conclusions ①The effect of leptin gene polymorphism on NAFLD is various in sex. ②Leptin levels in G allele frequencies were higher than A allele.