兔视神经损伤后视网膜神经节细胞MDA/SOD的变化

目的:观察兔视神经损伤后视网膜神经节细胞(retinal ganglion cell,RGC)丙二醛(malondialdehyde,MDA)/超氧化物歧化酶(superoxide dismutase,SOD)的变化。方法:选用新西兰白兔32只,分为对照组和损伤组,损伤组再根据损伤后不同时间分为3,7,14d组,每组8只16眼。采用硫代戊巴比妥酸法和黄嘌呤氧化法分别测定视网膜MDA/SOD含量。结果:损伤实验组MDA含量分别为5.95±0.78,7.67±0.64和8.29±1.02μmol/g,均明显高于正常对照组(3.82±0.54μmol/g);实验各组SOD含量分别为510±44,415±29和398±36μkat/g,均明显低于正常对照组(727±52μkat/g)。结论:自由基代谢在兔视神经损伤后RGC凋亡中起着重要作用,MDA/SOD含量测定在RGC凋亡的研究中有一定的意义。

Investigation of MDA/SOD in retinal ganglion cell after optic nerve injuries in rabbits

AIM:To study the changes of MDA/SOD in retinal ganglion cell(RGC) after optic nerve injuries in rabbits.METHODS:Thirty-two New Zealand rabbits without eye diseases were divided into two groups randomly:normal control group(n=8,16 eyes);injury group(n=24,48 eyes).The injury group was divided into three sub groups according to different time points:3-day group,7-day group and 14-day group(n=8,16 eyes,respectively).MDA/SOD was measured respectively by TBA and xanthine oxidase.RESULTS:The content of MDA in injury groups were 5.95±0.78,7.67±0.64 and 8.29±1.02μmol/g respectively,which was higher than the normal control group(3.82±0.54μmol/g).The content of SOD in injury groups were 510±44,415±29 and 398±36μkat/g respectively,which was lower than the normal control group(727±52μkat/g).CONCLUSION:Free radical metabolism plays an important role in RGC apoptosis after optic nerve injuries in rabbits and the determination of MDA/SOD had certain application value in the study of RGC apoptosis.