Diet-induced atherosclerosis inhibits release of noradrenaline from sympathetic nerves in rabbit arteries |
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| Institution: | 1. Division of Angiology, Servier Research Institute, 11, Rue des Moulineaux, 92150 Suresnes, France;2. Division of Pharmacology, Department of Medicine, Universitaire Instelling Antwerpen (UIA), University of Antwerp, Universiteitsplein 1, B2610, Wilrijk, Belgium;1. Department of Entomology, Chaudhary Charan Singh Haryana Agricultural University, Hisar 125001, India;2. Department of Plant Protection, College of Agricultural Engineering Science, University of Baghdad, Baghdad, Iraq;3. Directorate of Research, Chaudhary Charan Singh Haryana Agricultural University, Hisar 125001, India;4. Department of Genetics & Plant Breeding, Chaudhary Charan Singh Haryana Agricultural University, Hisar 125001, India;5. Department of Horticulture, Rajasthan Agricultural Research Institute, Durgapura, Jaipur, SKNAU, Jobner 302017, India;6. Faculty of Agriculture, Vivekanand Global University, Jaipur 302017, India;7. Department of Zoology, College of Science, King Saud University, Riyadh 11451, Saudi Arabia;8. Department of Biology and Environmental Engineering, University of Craiova, A.I.Cuza 13, Craiova 200585, Romania;9. College of Horticulture, Rajasthan Agricultural Research Institute, Durgapura, Jaipur, SKNAU, Jobner 302017, India;1. Department of Physiology, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensigh 2202, Bangladesh;2. Department of Soil Science, Faculty of Agriculture, Bangladesh Agricultural University, Mymensigh 2202, Bangladesh;3. Department of Pathology, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensigh 2202, Bangladesh;1. Natural Products Research Laboratory, Department of Pharmaceutical Sciences, School of Applied Sciences & Technology, University of Kashmir, Hazratbal, Srinagar, J&K, India 190006;2. Department of Pharmacognosy, College of Pharmacy, King Saud University, Riyadh, Saudi Arabia 11451;3. Pharmacognosy and Phytochemistry Research Laboratory, Department of Pharmaceutical Sciences, School of Applied Sciences & Technology, University of Kashmir, Hazratbal, Srinagar, J&K, India 190006 |
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| Abstract: | Contractile responses to sympathetic nerve stimulation and exogenous noradrenaline were compared in aortas and pulmonary arteries of control rabbits and rabbits fed a cholesterol-rich diet (0.3%) for 16 or 30 weeks. The diet-induced atherosclerosis reduced the contractions to increasing concentrations of exogenous noradrenaline (0.1 nM to 10 μM) in both arteries, and the reduction was more pronounced after 30 weeks of the hypercholesterolemia. The contractions produced with increasing frequencies of electrical stimulation (1–32 Hz) were nearly abolished in the atherosclerotic arteries. Labeling of the aorta and the pulmonary arteries with 3H]noradrenaline resulted in accumulation of radioactivity in both control and atherosclerotic blood vessels. After mounting the labeled blood vessels for superfusion, a basal efflux of 3H]noradrenaline and of 3H-metabolites was detected. In the atherosclerotic arteries, a decreased efflux of the intraneuronal deaminated metabolites 3,4-dihydroxyphenyl glycol (DOPEG) and 3,4-dihydroxymandelic acid (DOMA) was detected. Electrical stimulation at 1 Hz (pulmonary artery) or 2 Hz (aorta) caused an augmented efflux of total 3H from the control arteries; this was mostly due to release of intact 3H]noradrenaline. The electrical imp ulses evoked significantly less (16 weeks) or no (30 weeks) release of 3H]noradrenaline in the atherosclerotic arteries. These data illustrate that diet-induced atherosclerosis exerts an inhibitory action on the sympathetic nerve terminals in the aorta and the pulmonary artery of the rabbit. This effect, together with an inhibitory effect at the postjunctional level results in a loss of the responsiveness to nerve stimulation. The atherosclerotic process also inhibits the intraneuronal deamination of the sympathetic transmitter. |
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