Thyroidal stimulation of tubulin and actin in rat brain cytoskeleton |
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| Affiliation: | 1. Division of Pharmacy Practice, Faculty of Pharmaceutical Sciences, Ubon Ratchathani University, Ubon Ratchathani, Thailand;2. Department of Pharmacy, Siriraj Hospital, Mahidol University, Bangkok, Thailand;3. National Clinical Research Centre, Kuala Lumpur, Malaysia;4. School of Pharmacy, Monash University Malaysia, Selangor, Malaysia;5. Division of Cardiology, Department of Internal Medicine, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand;6. Clinical Pharmacy Division, Department of Pharmacy, Faculty of Pharmacy, Mahidol University, Bangkok, Thailand;7. Section for Clinical Epidemiology and Biostatistics, Faculty of Medicine, Ramathibodi Hospital, Mahidol University, Bangkok, Thailand;8. School of Epidemiology and Preventive Medicine, Monash University, Melbourne, VIC, Australia;9. School of Public Health, Curtin University, Perth, WA, Australia;10. Center of Pharmaceutical Outcomes Research (CPOR), Department of Pharmacy Practice, Faculty of Pharmaceutical Sciences, Naresuan University, Phitsanulok, Thailand;11. School of Pharmacy, University of Wisconsin, Madison, WI, USA;12. Asian Centre for Evidence Synthesis in Population, Implementation and Clinical Outcomes (PICO), Health and Well-being Cluster, Global Asia in the 21st Century (GA21) Platform, Monash University Malaysia, Bandar Sunway, Selangor, Malaysia;1. Bioprocess Engineering Department, Institute of Industrial and Environmental Biotechnology, National Institute of Genetic Engineering and Biotechnology (NIGEB), Tehran, Iran;2. Department of Pure Chemistry, Faculty of Chemistry, Shahid Beheshti University, G.C., Tehran, Iran;3. Nanobiotechnology Research Center, Avicenna Research Institute, ACECR, Tehran, Iran;4. Department of Chemistry, University of Zanjan, Zanjan, Iran;1. Dept. of Neuroscience Care and Society, Div. of Neurogeriatrics, Karolinska Institutet, 17164, Solna, Sweden;2. Department of Pharmaceutical Engineering & Technology, Indian Institute of Technology (BHU), Varanasi, 221005, India;3. Memory Clinic, Theme Aging, Karolinska University Hospital, 14186, Huddinge, Sweden;1. School of Pharmacy, Anhui Medical University, 81 Mei-Shan Road, Hefei 230032, China;2. The Key Laboratory of Anti-inflammatory and Immune Medicine, Ministry of Education, Anhui Medical University, Hefei, China;3. Anhui Provincial laboratory of inflammatory and immunity disease, Anhui Institute of Innovative Drugs, Hefei, China;4. Department of Laboratory Animal Science, Fudan University, Shanghai 200032, China;5. Department of Pharmacy, Hefei Fourth People''s Hospital, Hefei 230032, China;6. Psychopharmacology Research Laboratory, Anhui Mental Health Center, Hefei, China;7. Clinical Pharmacy, Affiliated Psychological Hospital of Anhui Medical University, Hefei, China;1. Department of Aging Science and Pharmacology, Faculty of Dental Science, Kyushu University, Fukuoka 812-8582, Japan;2. Center of Implant Dentistry, School of Stomatology, China Medical University, Shenyang 110002, China;3. The VIP Department, School of Stomatology, China Medical University, Shenyang 110002, China;4. Section of Geriatric Dentistry and Perioperative Medicine in Dentistry, Division of Maxillofacial Diagnostic and Surgical Sciences, Faculty of Dental Science, Kyushu University, Fukuoka 812-8582, Japan;5. Key Laboratory of Molecular Medicine and Biotherapy, School of Life Science, Beijing Institute of Technology, Beijing 100081, China;6. OBT Research Center, Faculty of Dental Science, Kyushu University, Fukuoka 812-8582, Japan;1. Department of Geriatrics, Hospital of Chengdu University of Traditional Chinese Medicine, Chengdu, 610072, China;2. Department of Geriatrics, Xi''an Hospital of Traditional Chinese Medicine, Xi''an, 710021, China;3. Department of Chinese Internal Medicine, Hospital of Chengdu University of Traditional Chinese Medicine, Chengdu, 610072, China |
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| Abstract: | In cultures of neonatal rat brain cells, labeled with 35S-methionine in the presence or absence of triiodothyronine (T3), the hormone promoted a significant enhancement of labeled tubulin and actin in the insoluble fraction (30,000 g pellet) of cell homogenate. To identify the specific sub-cellular fraction associated with this induction, organ cultures of 1 day rat cerebra were labelled with 35S-methionine in the presence and absence of T3 and the insoluble fraction (30,000 g pellet) was subfractionated into mitochondria, plasma membrane and cytoskeleton. Analysis of the labeled proteins by SDS-PAGE, autoradiography and densitometry revealed a T3-induced increase of 50–80% for both tubulin and actin, only in the cytoskeleton fraction without any significant effect on the other fractions. Similar results were obtained when plasma membrane or cytoskeleton were isolated directly from labeled cerebrum by conventional methods instead of fractionating from the 30,000 g pellet. Analysis of relative stimulation of labeled tubulin and actin by T3 in cytoskeleton fraction derived from primary cultures of neuronal (N) and glial (G) cells labeled with 35-methionine show that the stimulatory effect is predominantly on the N cells. Studies on the kinetics of induction of labeled tubulin and actin by T3 in the cytoskeleton fraction prepared from cerebra labeled with 35S-methionine for 2, 8 and 18 hrs revealed no significant difference at 2 hrs; at 8 hrs, an increased incorporation into both tubulin and actin was reproducibly seen in the controls relative to T3-treated samples. However by 18 hrs, this pattern reversed and an enhanced accumulation of both labeled tubulin and actin was observed under the influence of T3. The mechanism of this apparently intriguing effect of T3 on the kinetics of association of tubulin and actin with the cytoskeleton has been discussed in the light of the dual effect of the hormone on tubulin viz. enhancing its stability as well as rate of synthesis. The overall results indicate that the thyroid hormones play a major role in the cytoskeletal transport of tubulin and actin from their site of synthesis to that of assembly thus facilitating axodendritic outgrowth and morphological differentiation. |
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