Rapid purification of extracellular and intracellular Moloney murine leukemia virus |
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| Authors: | M. Aboud Marina Wolfson Yehudith Hassan M. Huleihel |
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| Affiliation: | (1) Microbiology and Immunology Unit, Faculty of Health Sciences, Ben-Gurion University of the Negev, Beer Sheva, Israel |
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| Abstract: | Summary The present study demonstrates the advantages of a combination of concentration by polyethylene glycol-6000 and Sepharose Cl-4B chromatography as a rapid procedure for retroviruses purification. This procedure can be completed within 3 hours, providing a high degree of virus purification with minimal damage to its structural and biological properties. Using transmission electron microscopy we observed many intracellular type-C virions in cytoplasmic vacuoles of 3T3/NIH cells chronically infected with Moloney murine leukemia virus. There intracellular virions could be isolated from postmitochondrial cytoplasmic fractions prepared from the infected cells by a procedure which minimized its contamination by extracellular free or membrane-bound virions. SDS-polyacrylamide gel electrophoresis showed that the intracellular and extracellular virions contained similar protein composition.With 5 Figures |
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