HIV-1B／C重组病毒感染者Gag特异性T淋巴细胞免疫应答的研究

目的研究中国主要流行的HIV-1B／C重组毒株感染者Gag特异性T淋巴细胞反应特征。方法本研究以10例感染时间〈1年和25例感染时间〉3年未接受抗病毒治疗的HIV-1B／C重组毒株感染者为研究对象,以10例HIV．1阴性健康人作为对照,用Elispot方法检测其针对HIV-1B／C同义BGag重叠多肽产生1干扰素的特异性T淋巴细胞反应。结果8例（8／10）感染时间〈1年的HIV-1B／C重组毒株感染者产生Gag特异性分泌Y干扰素的T淋巴细胞反应,主要识别散在分布的五条多肽;17例（68％）感染时间〉3年的感染者产生反应,主要识别p17区域内的-条和p24区域内六条多肽。感染时间〉3年组产生IFN-吖的特异性T淋巴细胞反应强度与病毒载量呈明显正相关（P=0．0318,r=0．519）,感染时间〈1年的感染者反应强度明显高于感染时间〉3年的感染者（P=0．021）。健康人对照组无阳性反应。结论HIV-1B／C重组病毒感染者在疾病进程不同阶段识别Gag的不同区域。

Analysis of gag-specific IFN-gamma secretion responses in HIV-1 B/C recombinant infectors

Objective To characterize the Gag-Specific T lymphocyte responses and identify immunodominant region recognized in Chinese HIV-1 recombinant subtype B/C infectors. Methods 10 antiretroviral treatment（ART） naive HIV-1 recombinant subtype B/C infectors with infected time in 1 year, 25 ART-naive infectors with infected time 〉 3 years and 10 HIV-1-seronegative healthy individuals were enrolled. HIV-1-speeifie T lymphocyte responses were analyzed by an IFN-γ Elispot assay against 123 overlapping peptides spanning HIV-1 Gag protein in the present study. Results Gag-specific T lymphocyte responses of interferon-gamma secretion were identified in 8 （8/10） Chinese HIV-1 recombinant subtype B/ C infectors with infected time in 1 year, the specific T lymphoeytes are mainly targeted at five seperated peptides. Responses were identified in 17 （68%） infectors with infected time more than 3 years, the specific T lymphoeytes are mainly targeted at one peptide in p17 and six in p24. There was obviously positive correlation（P = 0. 0318, r = 0. 519 ） between the magnitude of responses and viremia in infectors infected time 〉 3 years. The magnitude of response in infectors infected in 1 year was significantly higher than group infected time 〉 3 years（P = 0. 021 ）. None of healthy individuals produced positive responses. Conclusions HIV-1 recombinant subtype B/C Infectors at different stages of diseases recognize different region of gag.