PTEN蛋白在子宫内膜癌中的表达和预后关系的研究

目的:探讨子宫内膜腺癌和子宫正常组织及癌前病变组织中抑癌基因PTEN的表达,研究它的表达与子宫内膜腺癌的临床病理学特征及预后的关系。方法:采用免疫组化法,检测10例正常子宫内膜、12例单纯子宫内膜增殖、13例复杂子宫内膜增殖、20例不典型增生和74例子宫内膜腺癌组织中PTEN蛋白的表达,评价其与临床、病理因素和生存率的相关性。结果:抑癌基因PTEN在正常子宫内膜、单纯子宫内膜增殖、复杂子宫内膜增殖、不典型增生和子宫内膜腺癌中阳性表达率呈明显的下降趋势。在不典型增生和子宫内膜腺癌组中PTEN蛋白阳性表达均显著低于正常子宫内膜和单纯子宫内膜增生组(P<0.01)。癌组中PTEN阳性表达的缺失与组织学分级与临床手术分期及生存率有关(P<0.05),与肌层浸润深度无关(P>0.05)。结论:抑癌基因PTEN表达减少与子宫内膜病变的过程密切相关,PTEN蛋白的表达与临床、病理因素有关,对判断子宫内膜腺癌预后有意义。抑癌基因PTEN在子宫内膜癌及癌前病变中是有价值的分子标记物。

Prognosis and expression of tumor suppressor gene pten in adenocarcinoma endometrium

Objective:To study the prognosis and expression of tumor suppressor gene PTEN in adenocarcinoma endometrium or normal tissue or precancerosis and its clinicopathologica significance.Methods:.The expression of tumor suppressor gene PTEN was examined in normal endometrium(10 cases),simple endometrial hyperplasia(12 cases),multiplicity endometrial hyperplasia(13 cases),atypical hyperplasia(20 cases) and endometrioid adenocarcinoma(74 cases) using immunohistochemical technique,to evaluate its correlation in clinicopathologica and survival rate.Results:The expression level of PTENproteins was descending from normal endometrium,to simple endometrial hyperplasia,to multiplicity endometrial hyperplasia,to atypical hyperplasia,to endometrioid adenocarcinoma.The expression of PTEN proteins positively in atypical hyperplasia and endometrioid adenocarcinoma groups was significantly lower than these of normal endometrium and endometrial hyperplasia groups(P<0.01).In endometrioid adenocarcinoma the expression loss of PTEN protein positively was significantly associated with histological grade,clinical stage and survival rate(P<0.05).It is miscorrelates with muscular coat infiltrate(P>0.05).Conclusion:The results suggest that the loss expression of tumor suppressor gene PTEN is closely related on processing of endometrioid adenocarcinoma.The expression of PTEN protein correlates on clinicopathologiea and prognosis in endometrioid adenoearcinoma.PTEN is likely to be labeled molecules used to diagnose endometrial carcinoma and its precancerous lesion.