乙肝病毒相关性肾炎肾组织中HBVDNA分布的分子生物学检测

应用原位杂交（ＩＳＨ）技术观察１２例血清乙肝病毒（ＨＢＶ）标志物阳性的肾炎病人肾组织中ＨＢＶＤＮＡ的存在状况。结果表明，采用地高辛素（Ｄｉｇ）标记ＨＢＶＤＮＡ全长段探针ＩＳＨ时１０例肾组织中显示有ＨＢＶＤＮＡ。这１０例用Ｓ＋Ｃ段探针检测仍阳性者８例，ＨＢＶＤＮＡ最常见于肾小管上皮细胞内，呈核型或浆核型，其次是肾小球系膜区和毛细血管袢。ＬＳＡＢ免疫组化法示ＨＢｓＡｇ与ＨＢＶＤＮＡ的存在部位相吻合。提示ＨＢＶ引起的肾脏病变不仅是免疫介导的损害，亦可能有病毒直接侵犯，从分子病理水平为探讨ＨＢＶ相关性肾炎发病机理提供了新的依据。

Detection of HBV DNA in the Kidney in patients with hepatitis B virus-associated glomerulonephritis by molecular biology techniques

In situ hybridization(ISH) was used to detect the distribution of HBV DNA in the kidney in 12 pa-tients with positive serum HBV markers and glomerulonephritis. HBV DNA were detected in 10 out of the12 patients (83.3%)when ISH was done with a probe of full-length HBV DNA,and 8 out of 10(80.0%)with a probe of S+C segment HBV DNA. HBV DNA positive signals. were seen mostly in the nuclei orcytonuclei of the renal tubular cells and were seen in glomerular mesangial area and capillary tuft as well. The distribution of HBsAg detected by immunohistochemical stain(LSAB technique)coincides with that ofHBV DNA. It is suggested that the renal lesions due to HBV are not only the result of immunologic re-sponse,but also the outcome of direct invasion of HBV to this organ.This study provides new evidence onthe molecular mechanism for the research of the pathogenesis of HBV-related glomerulonephritis.