中药灯盏花对兔前颌骨缝牵张成骨的影响

目的:建立兔上颌骨缝牵张成骨实验模型,探讨灯盏花(EB)在上颌骨改建中的作用机制。方法:3月龄家兔随机分为对照组和实验组。牵引实验——16只兔(实验、对照各8只)佩戴自制前牵引面具,实验组持续前下方向牵引上颌骨28d,对照组不加力,拍摄X线头颅定位片,测量上颌骨矢状向的变化。用药实验——均行上颌骨前牵引。实验组前颌缝局部注射EB0.4ml/d,对照组NaCl0.4ml/d。行序列四环素荧光标记,测定28d后2组(共16只,每组8只)荧光标记带间的宽度;光镜下测定2组(共12只,每组6只)第7、14天TGF-β染色切片灰度值,采用SPSS10.0软件包进行成组t检验。结果:上颌骨相对于颅脑矢状方向的各项X线测量值实验组较对照组显著增大(P<0.05);荧光标记带间的平均距离实验组较对照组显著增宽(P<0.05);相同时间实验组较对照组TGF-β显著高表达(P<0.05);7d实验组较14d对照组TGF-β显著高表达(P<0.05);成骨活跃者成纤维细胞增多,血管分布增多;骨缝缘未见典型的活跃成骨细胞排列。结论:成功建立了前颌缝牵张成骨兔实验模型;EB具有促进骨组织形成的作用;血管生成、微循环改善、纤维成骨可能是兔上颌缝牵张的成骨机制之一;EB在兔前颌缝牵引成骨中可能具有直接成骨的作用。

Effect of Erigeron Breviscapine on premaxillary suture distraction osteogenesis in rabbit

PURPOSE: The aim of this study is to determine the effects of Erigeron Breviscapine (EB) in suture distraction osteogenesis (DO) in the maxillary protraction models of rabbit. METHODS: In distraction study, the rabbits were randomly divided into 2 groups based on protracted or unprotracted (n=16, 8 in each). The cephalograms were taken in the 0 and the 28th day. In EB study, all samples were protracted and were randomly divided into 2 groups based on injection of EB or NaCl. The width of serial tetracycline fluorochrome was labeled and measured during 28 days (n=16, 8 in each). In the 7th and the 14th day, the tissues of the premaxillary bone suture were harvested and processed for immunohistochemistry of TGF-beta (n=12, 6 in each). Group t test was performed by using SPSS10.0 software package. RESULTS: It was found that the cephalometric parameters of the sagittal maxilla were significantly increased in the protraction group after 28 days (P<0.05). The width of tetracycline fluorochrome was significantly increased in the EB group after 28 days (P<0.05). In the same time, there was stronger expression in EB group than in NaCl group (P<0.05). There was stronger TGF-beta expression in the 7th day EB group than in the 14th day NaCl group (P<0.05). There were abundant fibroblast and blood vessels in the active DO. There were not typical active osteoblasts in the border of the bone sutures. CONCLUSIONS: Maxillary protraction models of rabbit is successfully established. EB can accelerate DO. One reason of EB accelerating DO may be angiogenesis and improvement of microcirculation.Direct osteogenesis of EB is also possible. Supported by Science and Technology Development Project of Shanxi Province [Grant No.2002K11-G7(3)].