膀胱移行细胞癌患者D_(17)S_5位点基因频率分布特征分析

目的　探讨膀胱移行细胞癌患者D17S5位点的遗传多态性。方法　应用PCR、聚丙烯酰胺凝胶电泳及银染法 ,对 5 2例原发膀胱移行细胞癌患者进行了检测。结果　发现 10个等位基因位点 ,与正常对照群体的频率分布存在显著性差异 (P<0 0 0 1) ;基因型频率分布也与正常对照群体不同 ,不符合Hardy Weinberg平衡 (P <0 0 0 1) ;膀胱移行细胞癌患者纯合子的频率显著高于对照组 (P <0 0 1)。结论　膀胱移行细胞癌D17S5位点等位基因数目及基因型频率分布与对照人群不同 ,提示其频率分布的改变可能与等位基因的杂合性丢失有关

Distributing Analysis of the D_(17)S_5 Locus in Primary Bladder Transitional Cell Cancer

Objective To understand the genetic polymorphs of the D 17 S 5 locus in TCC.Methods The D 17 S 5 VNTR of 52 patients of TCC were observed by PCR,PAGE and silver staining methods.Results The analysis revealed 10 alleles,different from normal individuals significantly (P<0 001).The distribution of genotypes were in bad agreement with the expected number under the Hardy weinberg equilibrium(P<0 001).The heterozygosity of D 17 S 5 locus in TCC(61 54%) was less than that in normal organism(83 85%)(P<0 01).Conclusion The results suggested that the changes of the distribution of D 17 S 5 VNTR might be due to deletions of some frequencies or LOH.