莲房原花青素对D-半乳糖衰老小鼠脑组织抗氧化的作用

目的：探讨莲房原花青素（lotus seedpod procyanidins，LSPC）对D-半乳糖衰老小鼠脑组织抗氧化系统的影响。方法：雄性健康昆明种小鼠60只，随机分为对照组、衰老模型组、ESPC低剂量组（15mg·ml^-1）、LSPC中剂量组（30mg·ml^-1）和LSPC高剂量组（60mg·ml^-1）。采用小鼠皮下注射D-半乳糖（120mg·kg^-1·d^-1），连续注射8周，建立小鼠衰老模型，ISPC组造模同时灌胃给予ISPC，qd，连续给药8周。化学比色法检测5组小鼠脑组织中超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GSH—Px）和丙二醛（MDA）含量。结果：衰老模型组与对照组相比，小鼠脑组织SOD和GSH—Px活性明显降低，MDA含量明显增高（P〈0．01）。与衰老模型组相比较，LSPC能明显提高衰老小鼠脑组织中SOD活性和GSH—Px活性（P〈0．01），能减少MDA含量（P〈0．01）。结论：LSPC对D-半乳糖所致衰老小鼠具有明显的抗氧化作用。

Antioxidative Effects of LSPC in Brain Tissue of Senile Mice Induced by D-galactose

Objective ： To study the anti-oxidative effect of Lotus seedpod procyanidins （ LSPC ） in aging model mice. Method： Sixty healthy male Kunming mice were divided into five groups randomly： negative control group, aging group, low LSPC group（ 15 mg · ml^-1 ） , medium LSPC group （30 mg· ml^-1 ） and high LSPC group （60 mg· ml^-1 ）. The aging model was continually induced by subcutaneous injections of D-galactose（ 120 mg · kg^-1 · d^- 1 ） for 8 weeks in mice, LSPC was given by intragastric administration according to the grouping daily for 8 weeks during induction. The activities of superoxide dismutase （ SOD ） , glutathione peroxidase （ GSH- Px ） , and content of malonidaldelyde （MDA）of brain tissue were measured respectively by chemoehromatometry methods. Result： The SOD and GSH-Px activities in aging group were significantly decreased, and the content of MDA was significantly increased compared with negative control group（ P 〈 0.01 ）. Compared with aging group, the activities of SOD and GSH-Px of brain were increased significantly in low LSPC, medium LSPC and high LSPC groups （ P 〈 0.01 ）. The reduction of MDA was remarkably in low, medium and high KSPC groups （ P 〈 0.01 ）. Conclusion： LSPC has significantly antioxidations against aging mice induced by D-gajactose.