NS398对前列腺癌细胞PC-3小鼠体内致瘤性的抑制作用

目的探讨环氧化酶-2抑制剂NS398对前列腺癌细胞PC-3小鼠体内致瘤性的抑制作用及其机制。方法16只雄性裸小鼠建立负载前列腺癌细胞PC-3模型并随机分成2组,每组8只。对照组每只小鼠给予生理盐水0.2 mL,NS398治疗组每只小鼠给予NS398 3 mg.kg-1体质量,2组均腹腔注射,每周3次,共5周(15次)。测量裸鼠体质量、移植瘤的体积、瘤质量及抑瘤率,移植瘤组织标本进行HE染色观察形态学变化,应用免疫组织化学方法检测其微血管密度。结果治疗后NS398治疗组肿瘤质量明显低于对照组(P<0.01),而2组小鼠平均荷瘤体质量比较差异无统计学意义(P>0.05);NS398对小鼠前列腺癌的瘤质量抑制率为63.06%;NS398治疗组微血管密度值明显低于对照组(P<0.05)。结论NS398对前列腺癌PC-3移植瘤生长有抑制作用,可能与其抑制肿瘤新生血管生成有关。

Inhibitory effect of NS398 on oncogenicity of prostate carcinoma cells PC-3 in vivo

Objective To study the inhibitory effect of cyclooxygenase^-2 inhibiter NS398 on oncogenicity of PC-3 cells in vivo and to explore the mechanisms of the effect.Methods The prostate carcinoma models were established by inoculating PC-3 cells to subcutaneous tissue of mice.The models were randomly divided into two groups,eight mice in each group.The mice in control group were given 0.2 mL normal sodium,and the mice in NS398 group were given NS398 3 mg·kg^-1 by intraperitoneal injection,three times weekly for 5 weeks.The weight,volume and weight of transplantation tumor,and the rate of tumor inhibition were detected.HE staining was used to evaluate the morphological changes of prostate carcinoma tissue,the microvessel density（MVD） were detected by immunohistochemistry.Results The weight of tumors in NS398 group was significant less than that in control group（P〈0.01）,there was no significant difference in the body weight of mice between two groups（P〉0.05）.The inhibition rate of NS398 on the tumorous weight was 63.06%,the MVD was significantly decreased in NS398 group（P〈0.05）.Conclusion NS398 may inhibit PC-3 cell tumor growth in vivo by decreasing angiogenesis.