何首乌水溶性成分2,3,5,4′-四羟基二苯乙烯-2-O-β-D葡糖苷(ST I)对内皮细胞表达VEGF的影响何首乌水溶性成分2,3,5,4′-四羟基二苯乙烯-2-O-β-D葡糖苷(ST I)对内皮细胞表达VEGF的影响

目的研究何首乌水溶性成分2,3,5,4′-四羟基二苯乙烯-2-O-β-D葡糖苷(ST I)对溶血磷脂酰胆碱(LPC)诱导人脐静脉内皮细胞株ECV304细胞中血管内皮生长因子(VEGF)表达的影响。方法在ECV304细胞培养基中加入LPC(2.5 mg·L^-1)或LPC与ST I共孵24 h，收集各组条件培养基，用基础酶联免疫吸附试验(ELISA)检测各组条件培养基中VEGF蛋白含量；用原位杂交法、RT-PCR及Realtime RT-PCR法检测LPC对内皮细胞VEGF mRNA的表达及ST I的影响。结果ECV304细胞暴露于LPC后，VEGF蛋白分泌明显增加；加入ST I后VEGF蛋白含量明显降低; LPC可以使ECV304中VEGF mRNA的表达明显升高, 并使VEGF165 mRNA的表达升高；ST I可剂量依赖性地抑制VEGF165 mRNA的高表达。结论LPC能诱导ECV304细胞表达高水平的VEGF蛋白及VEGF mRNA，1×10^-5 mol·L^-1 ST I可抑制LPC的作用，降低VEGF的高表达。

Expression of VEGF in endothelial cells and the effects of 2, 3, 5, 4'-tetrahydroxystilbene-2-O-beta-D-glucoside]

AIM: To determine the effect of lysophosphatidylcholine (LPC) on the expression of vascular endothelial growth factor (VEGF) in human umbilical veins endothelial cell line (ECV304) and the inhibitory effect of 2, 3, 5, 4'-tetrahydroxystilbene-2-O-beta-D-glucoside (ST I) in vitro. METHODS: Exposure to 2.5 mg x L(-1) LPC or LPC + ST I for 24 hours, VEGF protein was determined by enzyme-linked immunosorbent assay (ELISA). Meanwhile, VEGF mRNA expression in ECV304 was examined by in situ hybridization. VEGF165 mRNA was examined by RT-PCR and Realtime RT-PCR. RESULTS: LPC upregulated VEGF protein and VEGF mRNA expression in the ECV304 cells. ST I was shown to markedly inhibit the LPC-induced increase of VEGF protein and VEGF165 mRNA (P < 0.001). CONCLUSION: LPC can induce a strong expression of VEGF in ECV304 cells and ST I can inhibit it.