非小细胞肺癌KAI1基因和TIMP-1、MMP-9表达与临床生物学行为的关系研究

目的探讨KAI1基因和基质金属蛋白酶抑制剂1(TIMP-1)、基质金属蛋白酶9(MMP-9)在非小细胞肺癌(NSCLC)中的表达,分析其与临床生物学行为的关系。方法应用免疫组化法检测73例NSCLC、51例相应的癌旁组织和13例非肿瘤性肺组织的KAI1和TIMP-1、MMP-9的表达,采用χ2检验和Pearson法分析三者与临床生物学行为的相关性。结果在73例NSCLC和64例癌旁及非肿瘤组织中,KAI1和TIMP-1的阳性率分别为39.7%、74.0%和90.6%、37.5%,两者比较差异有统计学意义(P0.05),而MMP-9阳性率为79.4%和67.1%,两者比较差异无统计学意义(P0.05)。在NSCLC组织中,KAI1检出率在不同TNM分期、淋巴结有无转移、不同肿瘤类型中差异有统计学意义(P0.05),TIMP-1、MMP-9的检出率在不同TNM分期、淋巴结有无转移中差异有统计学意义(P0.05),三者检出率与年龄、性别无关;KAI1的表达与TIMP-1、MMP-9呈负相关(r=-0.463 7、-0.344 5,P0.05)。结论 KAI1和TIMP-1、MMP-9均与肺癌的发展、转移有关,联合检测三者可成为评价NSCLC发生、发展的预后参考指标。

Research on the expressions of KAI1 gene,TIMP-1 and MMP-9 and their correlations with clinicopathologic features of non-small cell lung cancer

Objective To investigate the expressions of KAI1 gene,tissue inhibitor 1 of metalloproteinase （TIMP-1）and matrix metalloproteinase 9 （MMP-9）in non-small cell lung cancer （NSCLC）and their correlations with clinicopathologic features of NSCLC.Methods The expressions of KAI1 ,TIMP-1 and MMP-9 in 73 specimens of NSCLC tissues,51 specimens of corresponding adjacent tissues and 13 specimens of non-cancerous lung tissue were determined by immunohistochemistry.Their correlations with clinicopathologic features were analyzed by χ2 test and Pearson analysis.Results In 73 specimens of NSCLC tissues and 64 cases of corresponding adjacent and non-cancerous lung tissues,the positive rates of KAI1 and TIMP-1 were 39.7%,74.0% and 90.6%,37.5% with statistical significance （P 〈0.05 ）,and the positive rates of MMP-9 were 79.4%,67.1% without statistical significance （P〉0.05 ）.The expression of KAI1 was correlated to TNM stage,lymph node metastasis,histologic subtype and differentiation with statistical significance （P〈0.05）,and the TIMP-1 and MMP-9 were correlated to TNM stage and lymph node metastasis （P〈0.05）,but they were not correlated to age and sex.The expression of KAI1 was negatively correlated with the expressions of TIMP-1 and MMP-9 （r=-0.463 7,-0.344 5,P〈0.05）.Conclusions The KAI1 ,TIMP-1 and MMP-9 are related to the development of lung cancer.The combination determination of them will be the important prognosis reference indicator for the occurrence and development of NSCLS.