上海地区健康成年人外周血T淋巴细胞亚群参考区间调查

目的通过对上海地区333名健康成年人外周血T淋巴细胞亚群计数,调查正常人群T淋巴细胞亚群的参考区间,建立95%可信区间的参考区间,并分析和探讨其变化特点,为机体免疫状态的分析和临床诊疗提供参考。方法采用多参数三色免疫荧光标记外周血T淋巴细胞,使用BD FACSCalibur流式细胞仪检测并分析,获得T淋巴细胞亚群的百分比和绝对值。结果女性的CD3+百分比、CD4+/CD8+比值、CD3+CD4+百分比和CD4+CD45RA+百分比分别为(72.6%±8.1%)、1.57(0.74~4.70)、(44.8%±8.0%)和(1.71%±6.3%),均高于男性分别为(70.9%±8.5%)、1.43(0.51~4.12)、(40.6%±9.3%)和(15.1%±6.8%)。男性的淋巴细胞绝对值计数、CD8+CD28+的绝对值计数分别为(2 069.8±474.1)个/μL、(237.5±95.7)个/μL,均高于女性(1 932.8±469.7)个/μL、(218.1±111.1)个/μL。T淋巴细胞亚群的百分比在不同年龄组之间随年龄增大而发生变化,年龄相差越大,差异越大,其中50岁组与其他组别比较差异有统计学意义。333名健康成年人外周血淋巴细胞绝对值计数为(1 069.1~2 935.6)个/μL,T淋巴细胞亚群百分比计数为:CD3+细胞55.3%~87.6%,CD3+CD4+细胞25.2%~57.6%,CD3+CD8+细胞15.0%~42.1%,CD4+CD25+细胞2.5%~10.5%,CD4+CD45RA+细胞5.0%~27.2%,CD8+CD28+细胞4%~19.3%,CD4+/CD8+比值0.7~2.9。结论健康成年人T淋巴细胞亚群的百分比分布存在性别和年龄差异,由于这种差异不大,可以合并数据建立上海地区健康成年人外周血T淋巴细胞亚群的参考区间。

Investigation on the reference ranges of peripheral blood T lymphocyte subsets among healthy adults in Shanghai

Objective To establish the reference ranges of peripheral blood T lymphocyte subsets among 333 healthy adults in Shanghai,to analyze and discuss the change characteristics through the establishment of 95%confidence interval,and to provide the reference for immune status analysis and clinical diagnosis and treatment. Methods Multiparameter three-color immunofluorescence was used to stain the peripheral blood T lymphocytes,and BD FACSCalibur flow cytometry was used to acquire and analyze the percentages and absolute counts of T lymphocyte subsets.Results Among females,the CD3 ＋percentage,CD4 ＋/CD8 ＋ratio,CD3 ＋CD4 ＋percentage and CD4 ＋CD45RA＋percentage （72.6% ±8.1%）,1 .57（0.74 -4.70）,（44.8% ±8.0%）and （1 .71% ±6.3%）were respectively higher than those among males （70.9%±8.5%）,1 .43（0.51 -4.1 2）,（40.6%±9.3%）and （1 5.1%±6.8%）.The lymphocyte absolute count and CD8 ＋CD28 ＋absolute count in males （2 069.8 ±474.1 ）cells/μL and （237.5 ±95.7） cells/μL were higher than those in females （1 932.8 ±469.7 ） cells/μL and （21 8.1 ± 1 1 1 .1 ）cells/μL.The percentage of T lymphocyte subsets increased with the increase of age.There were statistical significances in 〉50 year-old group with the other groups.The absolute count of 333 healthy adults for peripheral blood T lymphocyte was （1 069.1 -2 935.6）cells/μL.The percentages of T lymphocyte subsets were：CD3 ＋55.3% -87.6%,CD3 ＋CD4 ＋25.2% -57.6%,CD3 ＋CD8 ＋1 5.0% -42.1%,CD4 ＋CD25 ＋2.5% -1 0.5%,CD4 ＋CD45RA＋5.0%-27.2 % and CD8 ＋CD28 ＋4%-1 9.3%,and CD4 ＋/CD8 ＋ratio was 0.7 -2.9.Conclusions There are differences for the percentage distribution of T lymphocyte subsets among healthy adults between sex and age. Since these differences can be ignored,the reference ranges of peripheral blood T lymphocyte subsets among healthy adults could be established in Shanghai.