山楂叶总黄酮对心肌缺血再灌注损伤大鼠心功能的影响

 目的观察山楂叶总黄酮(hawthornleavesflavonoids,HLF)对心肌缺血再灌注损伤大鼠的心功能保护作用。方法在结扎大鼠冠状动脉左前降支30min再灌注120min造成心肌缺血再灌注损伤模型,观察HLF对大鼠心功能、心肌酶学和脂质过氧化的影响。结果HLF能够对抗心肌缺血再灌注引起的左心室内压(LVSP)、左心室内压最大上升与下降速率(±dp/dtmax)、动脉血压的下降,并能够使心肌组织Na⁺-K⁺-ATP酶活性及丙二醛(MDA)含量降低和SOD的含量增高。结论HLF对大鼠心肌缺血再灌注引起的心功能减弱具有明显的保护作用,其机制可能与改善能量代谢障碍和抑制自由基生成或清除氧自由基作用有关。

Protective effects of hawthorn leaves flavonoids on cardiac function in rats suffered from myocardial ischemia reperfusion injury

OBJECTIVE To investigate the protective effects of hawthorn leaves flavonoids (HLF) on cardiac function in rats suffered from myocardial ischemia reperfusion injury.METHODS Myocadial ischemia reperfusion injury model was established by the ligation of left desending coronary artery for 30 min and reperfusion for 120 min in rats, and the influence of HLF on cardiac function, enzymes, lipid peroxide of myocardium was observed.RESULTS HLF attenuated the declines of LVSP and ± dp/dtmax and blood pressure in arterial, decreased Na⁺ -K⁺-ATPase activity and the level of malondialdehyde( MDA) , and increased superoxide dismutase( SOD) activity in ischemic myocardium . CONCLUSION The results suggested that the effects of HLF on cardiac function in the process of ischemia reperfusion may be related to the mechanism of improving energy metabolism, scavarging the oxygen free radicals and inhibiting production of free rascals in ischemic myocardium.