| 川芎嗪对内皮祖细胞的功能影响 |
| |
| 引用本文： | 林杰,徐力辛,杨德业,黄伟剑,林捷,张怀勤.川芎嗪对内皮祖细胞的功能影响[J].浙江中医药大学学报,2008,32(5). |
| |
| 作者姓名： | 林杰 徐力辛 杨德业 黄伟剑 林捷 张怀勤 |
| |
| 作者单位： | 1. 温州医学院附属第一医院,温州,325000
2. 温州医学院心血管生物和基因研究所 |
| |
| 摘 要： | 目的]观察川芎嗪（TMP）对体外培养内皮祖细胞（EPCs）功能影响和损伤保护作用，探讨它对冠心病的治疗作用。方法]密度梯度离心法获取脐血单个核细胞，培养7天后，收集贴壁细胞，流式细胞仪、荧光显微镜法、matrigel试剂鉴定EPCs。收集贴壁细胞并分组为正常对照组，TMP（5mg／L、25mg／L100mg／L、200mg／L）干预组，H2O2（500μmol／L）氧化应激模型组，TMP（5mg／L、25mg／L、100mg／L、200mg／L）和H2O2共同干预组，培养24h。分别观察EPCs增殖、粘附能力及凋亡率。结果]与正常对照组相比，（5mg／L、25mg／L、100mg／L）TMP干预组对脐血来源的EPCs增殖、粘附功能无显著影响，（200mg／L）TMP干预组显著抑制脐血来源的EPCs增殖、粘附；与H202氧化应激损伤组相比，（5mg／L、25mg／L、100mg／L）TMP能显著降低H2O2对EPCs增殖、粘附抑制作用及细胞凋亡。结论]低浓度TMP能保护EPCs氧化应激损伤，改善增殖、粘附能力，减少细胞凋亡，但对正常培养的EPCs增殖和粘粘功能无显著的促进或抑制作用。
|
| 关 键 词： | 川芎嗪 内皮祖细胞 过氧化氢 缺血性疾病 冠心病 |
Effect of Tetramethylpyrazine on the Function of Endothelial Progenitor Cells in vitro |
| |
| Lin Jie,Xu Lixin,Yang Deye,et al First Affiliated Hospital of Wenzhou Medical College,China.Effect of Tetramethylpyrazine on the Function of Endothelial Progenitor Cells in vitro[J].Journal of Zhejiang University of Traditional Chinese Medicine,2008,32(5). |
| |
| Authors: | Lin Jie Xu Lixin Yang Deye First Affiliated Hospital of Wenzhou Medical College China |
| |
| Institution: | Lin Jie,Xu Lixin,Yang Deye,et al First Affiliated Hospital of Wenzhou Medical College(325000),China |
| |
| Abstract: | Objective] This study was to investigate the effect of TMP on function of EPCs in normal or hydrogen peroxide(H2O2)inducing injury model cell culture,and to approach a therapy to coronary heart disease(CHD).Method] Total mononuclear cells were isolated from the cord blood by ficoll density gradient centrifugation,cultured in vitro.After the cells cultured for 7 days,identified them by cellmarkers and the ability of intaking the ac-LDL,and made sure they were EPCs.To divide the cells into three groups,TMP(... |
| |
| Keywords: | tetramethylpyrazine endothelial progenitor cell hydrogen peroxide ischemic disease coronary heart disease |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
