| 慢性酒精刺激对大鼠海马突触素ⅠmRNA表达的影响 |
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| 引用本文： | 刘丹,李昌琪,伍校琼,卢大华,罗学港.慢性酒精刺激对大鼠海马突触素ⅠmRNA表达的影响[J].中国现代医学杂志,2003,13(24):5-7,10. |
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| 作者姓名： | 刘丹 李昌琪 伍校琼 卢大华 罗学港 |
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| 作者单位： | 中南大学湘雅医学院人体解剖学与神经生物学系,湖南,长沙,410013 |
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| 基金项目： | ThisworkwassupportedbyNaturalScienceGrantofHunanProvince(NO :0 2JJY3 0 2 2 ) |
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| 摘 要： | 目的 探讨慢性酒精刺激对大鼠海马突触素I(synapsin I)mRNA表达的影响。方法 设立正常对照组(即纯水组)与实验组，两组动物分别以纯水、10％酒精为唯一饮料连续喂养，两组动物又分30d与60d两组。用组织原位杂交技术观察海马突触素Ⅰ mRNA表达的变化。结果 正常对照组大鼠海马CA各区及齿状回有强的突触素Ⅰ mRNA表达。慢性酒精刺激30d后突触素Ⅰ mRNA在海马的表达与正常对照组相比有显著下降。慢性酒精刺激60d后突触素Ⅰ mRNA在海马的表达与30d相比进一步下降。结论 慢性酒精中毒引起大鼠海马突触素ⅠmRNA表达的下降，其表达的下降可能与学习记忆障碍有关。
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| 关 键 词： | 酒精 突触素I 海马 组织原位杂交 大鼠 |
Effect of chronic ethanol consumption on expression of synapsin Ⅰ Mrna in rat hippocampus |
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| LIU Dan,LI Chang-qi,WU Xiao-qiong,LU Da-hua,LUO Xue-gang.Effect of chronic ethanol consumption on expression of synapsin Ⅰ Mrna in rat hippocampus[J].China Journal of Modern Medicine,2003,13(24):5-7,10. |
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| Authors: | LIU Dan LI Chang-qi WU Xiao-qiong LU Da-hua LUO Xue-gang |
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| Abstract: | Objective:To study the effect of chronic ethanol stimulation on the expression of synapsin Ⅰ messenger RNA in the rat hippocampus.Methods:Rats were divided into two groups:pure water or 10% ethanol was as absolute beverage to breed for 30 days and 60 days.The synapsin Ⅰ mRNA in the hippocampus was observed by in situ hybridization histochemistry.Results:Intersive synapsin Ⅰ mRNA labeling was observed in the hippocampus CA regions and dentate gyrus.The synapsin Ⅰ mRNA expression was decreased markedly after ethanol treatment for 30 days,and there was progressively decline of synapsin Ⅰ mRNA expression after ethanol treatment for 60days.Conclusions:Chronic ethanol treatment can decline the expression of synapsin Ⅰ mRNA in the rat hippocampus. |
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| Keywords: | ethanol hippocarnpus in situ hybridization histochemistry rat |
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