N乙L-半胱氨酸对胎儿卵巢组织体外培养的影响

目的：探讨硫醇类抗氧化剂 N 乙酰 L 半胱氨酸(n acetyl l cysteine，NAC)对胎儿卵巢组织体外培养效果的影响。方法：取中期引产的20～28周死亡女胎的卵巢，分别在含NAC 25、50、100?mmol/L(实验组)和不含NAC(对照组)的培养液内培养0～9?d，采用液相平衡竞争放射免疫分析法检测各组卵巢组织在不同的培养时间雌二醇（estrogen，E2）分泌量；观察培养后各组卵巢卵泡的形态学变化。结果：培养后卵泡可进一步发育，各期卵泡构成比实验组与未培养组、对照组比较差异均有统计学意义(P<0.05)，而3个不同剂量实验组两两之间在卵泡构成比上差异无统计学意义（P>0.05）；胎儿卵巢组织经体外培养后能够分泌E2，E2分泌量实验组和对照组之间差异有统计学意义(P<0.05)，随着培养时间的延长E2分泌量显著增加(P<0.05)；而在添加不同剂量NAC的3个实验组，两两比较差异则无统计学意义(P>0.05)。结论：在培养液中添加NAC后，E2分泌量增加，各期卵泡的形态得以维持并能进一步发育，为人胎儿卵巢培养后移植提供了实验依据。

Effects of N-acetyl-L-cysteine on the fetal ovarian tissues cultured in vitro

To investigate the effects of antioxidant N acetyl L cysteine(NAC) on the fetal ovarian tissues cultured in vitro. Methods: Fetal ovaries of 20 to 28 weeks were cultured for 0 to 9 days in 4 NAC groups: 25?mmol/L, 50?mmol/L, 100?mmol/L and 0?mmol/L. The concentrations of estrogen in the culture medium were measured by radio immunoassay and the morphologic changes of the cultured ovarian tissues were observed. Results: The proportion of matured follicles of different stages in the 3 experimental groups were significantly different compared with the control group (P<0.05) and the estrogen output in both control and experimental groups were significantly increased with the time prolonged(P＜0.05), however, the differences among the experimental groups were not significant　(P>0.05); the estrogen output of the fetal ovarian tissues in the 3 experimental groups were significantly increased than that in the control group (P<0.05), however, the differences among the experimental groups were not significant (P>0.05). Conclusion: N acetyl L cysteine can increase the estrogen output and the proportion of mature follicles in the fetal ovarian tissues cultured in vitro.