MicroRNA let-7a Ameliorates Con A-Induced Hepatitis by Inhibiting IL-6-Dependent Th17 Cell Differentiation |
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Authors: | Yingying Zhang Xiangmin Wang Min Zhong Mengying Zhang Qifeng Suo Kun Lv |
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Institution: | 1. Laboratory of Medicine of Yijishan Hospital, Wannan Medical College, Wuhu, 241001, People’s Republic of China 2. Department of Pathology of Yijishan Hospital, Wannan Medical College, Wuhu, 241001, People’s Republic of China 3. Central Laboratory of Yijishan Hospital, Wannan Medical College, Wuhu, 241001, People’s Republic of China
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Abstract: | In this study we explored the effects of microRNA let-7a on Con A-induced hepatitis and its possible mechanisms involved. We demonstrated that IL-6 and IL-17 expression were significantly upregulated in the liver following Con A treatment and IL-6 level was correlated with the IL-17 expression. To explore whether let-7a may have therapeutic effect on Con A-induced hepatitis, mice was infected with a lentiviral vector containing the let-7a sequence 7 days before Con A treatment. Significantly reduced Th17 cells and remarkably increased regulatory T cells frequency in the liver tissue were found as compared to control mice. It was accompanied by a significant decreased level of inflammatory cytokines as TNF-α, IL-6 and IFN-γ in the serum, and an decreased level of Th17 lineage-specific genes such as Il17a, Il17f, Il21 and Il23r. let-7a was further found to inhibit Th17 differentiation by downregulating IL-6 secretion. It may represent as a novel therapeutic strategy in treating immune-mediated inflammatory hepatitis. |
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