慢性HBV感染前C区变异与病毒复制水平关系

探讨HBV前C基因变异与病毒复制水平的关系在慢性HBV感染者中的意义。应用错配聚合酶链反应（PCR）－限制性片段长度多态性（RFLP）分析与荧光定量聚合酶链反应检测HBVDNA相结合，对30例HBsAg（＋）、抗－HBe( )及抗－HBc( )慢性HBV感染者，其中无症状携带者（AsC)9例、慢性乙型肝炎（CHB）12例及慢性重症肝炎（CHF）9例进行前C区基因变异与HBVDNA水平关系进行分析。AsC组3例（33．33％），CHB组9例（75％）及CHF组8例（88．89％）有A83(nt1896)变异。荧光定量PCR结果表明HBVDNA含量在CHF组中最高。HBV前C变异与HBV不同感染状态中都可见，其病毒复制水平与肝病活动相关。

Relationship of HBV precore mutation with serum HBV DNA in the patients with chronic Hepatitis B Virus infection

To study the relationship of HBV precore gene mutation with serum HBV DNA in the patients with chronic HBV infection.In 30 patients with HBsAg(+)/anti HBe(+)and anti HBc(+)(9 cases with asymptomatic HBsAg carrier(AsC),12 cases with chronic hepatitis B(CHB)and 9 cases with chronic fulminant hepatitis B(CHF)the precore mutation were analyzed by combining restriction fragment length polymorphism and an amplisensor assay for quantification of HBV DNA.The detection rates of mutant (nt 1896G-A)in AsC,CHB and CHF were3(33.33%),9(75.00%)and 8(88.89%)respectively.There were more core gene mutations in CHF and CHB than in AsC.Precore gene mutations exist in all different HBV infected patients.The replication level of HBV is possible related to the active of liver disease.