血液稀释对局部脑缺血大鼠脑血流及脑梗死的影响

为探讨中度急性等容血液稀释 (ANHD)对大鼠局部脑缺血模型的脑血流和脑梗死面积的影响 ,将Wistar脑缺血模型大鼠随机分为对照组 (n =8)和ANHD组 (n =8) ,于缺血前和缺血 1 2 0min内 ,用激光多普勒血流仪连续测定缺血周边皮质的局部脑血流 (LCBF)变化 ,缺血后 2 4h测定脑梗死面积。结果显示 2组在脑缺血后LCBF均显著下降 (P <0 .0 1 )。ANHD组下降的幅度显著小于对照组 (P <0 .0 1 ) ,并且缺血 2 4h后的脑梗死面积也显著小于对照组 (P <0 .0 5或P <0 .0 1 )。提示中度ANHD可增加大鼠局部脑缺血模型的缺血周边区的LCBF ,缩小脑梗死面积 ,在脑缺血情况下具有脑保护作用

Effects of Hemodilution on Cerebral Blood Flow and Infarction Size in Rat Model of Focal Cerebral Ischemia

In order to assess the effects of moderate acute normovolemic hemodilution (ANHD) on cerebral blood flow and infarction size in a rat model of focal cerebral ischemia, sixteen Wistar rats were randomly assigned into two groups: control group ( n =8) and ANHD group ( n =8). The Hct of the animals in ANHD group was reduced to 30.7%. Cortical local cerebral blood flow (LCBF) was monitored in both groups before and during the 120 min occlusion of the left middle cerebral artery with laser Doppler flowmetry. The infarction size of each brain was determined at 24 h after ischemia. LCBF significantly decreased in both groups ( P <0.01). The decreasing degree of the ANHD group was significantly lower than that of the contral group ( P <0.01), and its cerebral infarction size was significantly smaller than that of the control group( P <0.05 or P <0.01). The results indicate that moderate ANHD increases cortical LCBF and decreases cerebral infarction size in a rat model of focal ischemia. It has a protective effect on cerebral ischemia.