慢性浅表性胃炎不同程度脾胃湿热证与胃黏膜水通道蛋白3、4基因表达的相关性

目的：探讨慢性浅表性胃炎（chronic superficial gastritis, CSG）不同程度脾胃湿热证与胃黏膜水通道蛋白（aquaporin, AQP）3、4基因表达的相关性。 方法：共纳入24例CSG患者，按脾胃湿热证不同程度分为轻、中、重度组，另选择8名健康人作为正常对照。胃镜下取胃体上部黏膜，液氮保存，荧光定量聚合酶链反应法检测胃黏膜AQP3和AQP4的基因表达。 结果：中、重度脾胃湿热证组AQP3和AQP4基因表达水平均高于正常对照组和轻度脾胃湿热证组（P〈0.05，P〈0.01）。重度脾胃湿热证组AQP3高于中度脾胃湿热证组（P〈0.05）。 结论：脾胃湿热程度不同，胃黏膜AQP3和AQP4基因表达亦不同，脾胃湿热程度与胃黏膜AQP3和AQP4基因表达相关，其表达会随着湿热程度的加重而升高。

Relationship between gene expressions of aquaporin 3 and 4 and various degrees of spleen-stomach dampness-heat syndrome in chronic superficial gastritis

Objective: To explore the relationship between gene expressions of aquaporin (AQP) 3 and 4 and various degrees of spleen-stomach dampness-heat syndrome in chronic superficial gastritis (CSG).Methods: Twenty-four CSG patients were divided into three groups according to the degrees of spleen-stomach dampness-heat syndrome: mild, moderate and severe groups. There were 8 patients in each group, and another 8 healthy persons were selected as normal control. Samples of mucosa of upper stomach in the included patients and normal persons were collected by gastroscopy, and then were stored in liquid nitrogen. The gene expressions of AQP3 and AQP4 in gastric tissue were examined by fluorescence quantitative polymerase chain reaction.Results: The gene expressions of AQP3 and AQP4 in the moderate and severe groups were higher than that in the mild group and normal group (P<0.05, P<0.01). The gene expression of AQP3 in the severe group was higher than that in the moderate group (P<0.05). Conclusion: The gene expressions of AQP3 and AQP4 in gastric mucosa are different in patients with various degrees of spleen-stomach dampness-heat syndrome of CSG. There is a relationship between expressions of AQP3 and AQP4 genes and the degrees of spleen-stomach dampness-heat syndrome, and the gene expressions will increase with the aggravation of the dampness-heat syndrome.