TZT-1027 elucidates antitumor activity through direct cytotoxicity and selective blockade of blood supply |
| |
Authors: | Hashiguchi Naoko Kubota Tetsuro Koh Jun-Ichi Yamada Yoshinori Saikawa Yoshiro Otani Yoshihide Watanabe Masahiko Kumai Koichiro Kitajima Masaki Watanabe Jun-Ichi Kobayashi Motohiro |
| |
Institution: | Department of Surgery, School of Medicine, Keio University, Tokyo 160-8582, Japan. |
| |
Abstract: | BACKGROUND: TZT-1027 is a newly developed antitumor agent derived from dolastatin 10. MATERIALS AND METHODS: The in vitro activity of TZT-1027 on MCF-7 and R-27 cells was evaluated by MTT assay. TZT-1027 1 mg/kg/week was administered i.v. for 4 weeks into nude mice bearing MCF-7 and R-27. Subsequently, primary cultured cells from xenografts were also used for CD-DST. Two mg of TZT-1027 or 40 mg docetaxel per kg were injected i.v. into nude mice bearing R-27. 0.2% Evans blue was injected to assess the blood flow. RESULTS: TZT-1027 suppressed the in vitro growth of MCF-7 cells, while R-27 cells were resistant to TZT-1027, although its in vivo antitumor activity was remarkable. TZT-1027 blockaded R-27 tumor blood flow immediately after injection; blood flow was not affected by docetaxel. CONCLUSION: TZT-1027 exerts its antitumor activity through direct cytotoxicity against MCF-7 cells and through selective blockade of tumor blood flow against R-27 cells. |
| |
Keywords: | |
本文献已被 PubMed 等数据库收录! |
|