miR-124调控SOS1表达及对U87胶质瘤细胞增殖的影响

目的:探讨miR-124对胶质瘤细胞增殖的抑制作用及作用靶点.方法:应用生物信息整合分析,SOS1是miR-124负向调节胶质瘤细胞的靶点.应用细胞转染和荧光素酶检测分析证实miR-124对SOS1的作用关系.结果:SOS1是miR-124作用靶点,miR-124直接作用于SOS1 mRNA 3'端非编码区(UTR),但不作用于突变型的3(UTR).miR-124通过作用于靶点SOS1抑制了体外胶质瘤细胞的增殖.结论:SOS1在恶性胶质瘤细胞中呈正向调控,miR-124的含量上升可导致SOS1含量下降,miR-124通过调节SOS1/Raf/ERK信号通路在抑制细胞生长中起重要作用.

miR-124 regulates the expression of SOS1 in glioblastoma and has effects on proliferation of U87 glioblastioma cells

Objective:Confirming SOS1 gene is a target of miR-124 gene regulating glioma cells,miR-124 can directly act on the 3'untranslated region (UTR) of SOS1 mRNA,miR-124 silence SOS1 inhibition of glioma cell proliferation.Methods:Using bioinformatics integration analysis,SOSI was the target of miR-124 negative regulation of glioma cells.Cell transfection and luciferase assay confirmed the role of miR-124 in SOS1.Results:SOS1 was the target of miR-124.It was confirmed that miR-124 directly acted on the 3'untranslated region (UTR) of SOS1 mRNA,but not on mutant 3(UTR).miR-124 inhibited the proliferation of glioma cells by acting on target SOS1.Condusion:SOS1 is positively regulated in malignant glioma cells,and our study demonstrates that upregulation miR-124 is responsible for the downregulation of SOS1 in GBM,and miR-124 has an important role in inhibiting cell growth by regulating the SOS1/Raf/ERK signaling pathway.