IGF-1对胃癌血红素氧合酶-1表达的影响

目的:研究胃癌患者血清中胰岛素样生长因子-1(IGF-1)和血红素氧合酶-1(HO-1)的表达相关性,并探讨IGF-1对胃癌细胞中HO-1表达的调控作用.方法:收集胃癌患者血清标本,ELISA检测血清中IGF-1和HO-1的含量;胃癌细胞株SGC7901作为体外实验载体,不同浓度IGF-1干预胃癌细胞,Real time PCR和Western blot检测胃癌细胞中HO-1 mRNA和蛋白的表达量;随后在胃癌细胞培养基中加入IGF-1阻断剂,观察应用阻断剂前后胃癌细胞HO-1表达的变化.结果:胃癌患者血清中IGF-1和HO-1的表达较正常人群明显升高,且两者的表达呈正相关性.体外培养胃癌细胞SGC7901,IGF-1可显著促进胃癌细胞HO-1的表达,且该效应呈浓度依赖性.培养基中应用IGF-1阻断剂后,胃癌细胞HO-1的表达量明显受到抑制.结论:本实验探讨胃癌中IGF-1和HO-1表达的相关性,初步阐述IGF-1对胃癌HO-1表达的正向调控作用,为HO-1参与IGF-1促进胃癌发生及发展提供了理论依据.

IGF-1 regulates the expression of HO-1 in gastric cancer

Objective:To investigate the relationship between the expressions of insulin-like growth factor-1(IGF-1) and hemeoxygenase-1(HO-1) in patients with gastric cancer.To study the effects of IGF-1 on the expression of HO-1 in gastric cancer cells.Methods:We collect serum samples of patients with gastric cancer,then,ELISA was used to detect the levels of IGF-1 and HO-1 expression.Gastric cancer cells SGC7901 was used in vitro,which were intervened by IGF-1 in different concentrations.The mRNA and protein expression of HO-1 in gastric cancer cells were detected by RT-PCR and Western blot analysis.Then the IGF-1 blockers were added to the culture medium of gastric cancer cells,to observe the change of the expression of HO-1 in gastric cancer cells both before and after using blockers.Results:The expressions of IGF-1 and HO-1 in the patients with gastric cancer significantly were increased than those in the controls.The significant positive correlations were observed between the levels of IGF-1 and HO-1.Gastric cancer cells SGC7901 were cultured in vitro,and the HO-1 expression in gastric cancer cells was significantly enhanced by IGF-1 by a concentration-dependent manner.The HO-1 expression in gastric cancer cells was significantly inhibited by IGF-1 blockers.Conclusion:This experiment studies the correlation of IGF-1 and HO-1 in gastric cancer,and suggests the positive regulatory effects of IGF-1 on HO-1 expression in gastric cancer cells.Our research provides the theoretical basis for gastric cancer.